Details

Autor(en) / Beteiligte

• Li, Xuejie
• Pan, Lijie
• Wang, Bin
• Pan, Li

Titel

The Histone Deacetylases HosA and HdaA Affect the Phenotype and Transcriptomic and Metabolic Profiles of Aspergillus niger

Ist Teil von

• Toxins, 2019-09, Vol.11 (9), p.520

Ort / Verlag

Switzerland: MDPI AG

Erscheinungsjahr

2019

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Histone acetylation is an important modification for the regulation of chromatin accessibility and is controlled by two kinds of histone-modifying enzymes: histone acetyltransferases (HATs) and histone deacetylases (HDACs). In filamentous fungi, there is increasing evidence that HATs and HDACs are critical factors related to mycelial growth, stress response, pathogenicity and production of secondary metabolites (SMs). In this study, seven histone deacetylase-deficient strains were constructed to investigate their effects on the strain growth phenotype as well as the transcriptomic and metabolic profiles of secondary metabolic pathways. Phenotypic analysis showed that deletion of in FGSC A1279 leads to a significant reduction in growth, pigment production, sporulation and stress resistance, and deletion of leads to an increase in pigment production in liquid CD medium. According to the metabolomic analysis, the production of the well-known secondary metabolite fumonisin was reduced in both the and mutants, and the production of kojic acid was reduced in the mutant and slightly increased in the mutant. Results suggested that the histone deacetylases HosA and HdaA play a role in development and SM biosynthesis in FGSC A1279. Histone deacetylases offer new strategies for regulation of SM synthesis.