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The Histone Deacetylases HosA and HdaA Affect the Phenotype and Transcriptomic and Metabolic Profiles of Aspergillus niger
Ist Teil von
Toxins, 2019-09, Vol.11 (9), p.520
Ort / Verlag
Switzerland: MDPI AG
Erscheinungsjahr
2019
Quelle
Free E-Journal (出版社公開部分のみ)
Beschreibungen/Notizen
Histone acetylation is an important modification for the regulation of chromatin accessibility and is controlled by two kinds of histone-modifying enzymes: histone acetyltransferases (HATs) and histone deacetylases (HDACs). In filamentous fungi, there is increasing evidence that HATs and HDACs are critical factors related to mycelial growth, stress response, pathogenicity and production of secondary metabolites (SMs). In this study, seven
histone deacetylase-deficient strains were constructed to investigate their effects on the strain growth phenotype as well as the transcriptomic and metabolic profiles of secondary metabolic pathways. Phenotypic analysis showed that deletion of
in
FGSC A1279 leads to a significant reduction in growth, pigment production, sporulation and stress resistance, and deletion of
leads to an increase in pigment production in liquid CD medium. According to the metabolomic analysis, the production of the well-known secondary metabolite fumonisin was reduced in both the
and
mutants, and the production of kojic acid was reduced in the
mutant and slightly increased in the
mutant. Results suggested that the histone deacetylases HosA and HdaA play a role in development and SM biosynthesis in
FGSC A1279. Histone deacetylases offer new strategies for regulation of SM synthesis.