Details

Autor(en) / Beteiligte

• Nguyen, Trang Thi
• Huy, Tran Xuan Ngoc
• Aguilar, Ched Nicole Turbela
• Reyes, Alisha Wehdnesday Bernardo
• Salad, Said Abdi
• Min, Won-Gi
• Lee, Hu-Jang
• Kim, Hyun-Jin
• Lee, John-Hwa
• Kim, Suk

Titel

Intracellular Growth Inhibition and Host Immune Modulation of 3-Amino-1,2,4-triazole in Murine Brucellosis

Ist Teil von

• International journal of molecular sciences, 2023-12, Vol.24 (24), p.17352

Ort / Verlag

Switzerland: MDPI AG

Erscheinungsjahr

2023

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Catalase, an antioxidant enzyme widely produced in mammalian cells and bacteria, is crucial to mitigating oxidative stress in hostile environments. This function enhances the intracellular survivability of various intracellular growth pathogens, including ( .) . In this study, to determine whether the suppression of catalase can inhibit the intracellular growth of . , we employed 3-amino-1,2,4-triazole (3-AT), a catalase inhibitor, in both RAW 264.7 macrophage cells and an ICR mouse model during infection. The intracellular growth assay indicated that 3-AT exerts growth-inhibitory effects on within macrophages. Moreover, it contributes to the accumulation of reactive oxygen species and the formation of nitric oxide. Notably, 3-AT diminishes the activation of the nucleus transcription factor (NF-κB) and modulates the cytokine secretion within infected cells. In our mouse model, the administration of 3-AT reduced the proliferation within the spleens and livers of infected mice. This reduction was accompanied by a diminished immune response to infection, as indicated by the lowered levels of TNF-α, IL-6, and IL-10 and altered CD4 /CD8 T-cell ratio. These results suggest the protective and immunomodulatory effects of 3-AT treatment against infection.