Details

Autor(en) / Beteiligte

• Jenkins, Edward
• Körbel, Markus
• O'Brien-Ball, Caitlin
• McColl, James
• Chen, Kevin Y
• Kotowski, Mateusz
• Humphrey, Jane
• Lippert, Anna H
• Brouwer, Heather
• Santos, Ana Mafalda
• Lee, Steven F
• Davis, Simon J
• Klenerman, David

Titel

Antigen discrimination by T cells relies on size-constrained microvillar contact

Ist Teil von

• Nature communications, 2023-03, Vol.14 (1), p.1611-1611, Article 1611

Ort / Verlag

England: Nature Publishing Group

Erscheinungsjahr

2023

Quelle

MEDLINE

Beschreibungen/Notizen

• T cells use finger-like protrusions called 'microvilli' to interrogate their targets, but why they do so is unknown. To form contacts, T cells must overcome the highly charged, barrier-like layer of large molecules forming a target cell's glycocalyx. Here, T cells are observed to use microvilli to breach a model glycocalyx barrier, forming numerous small (<0.5 μm diameter) contacts each of which is stabilized by the small adhesive protein CD2 expressed by the T cell, and excludes large proteins including CD45, allowing sensitive, antigen dependent TCR signaling. In the absence of the glycocalyx or when microvillar contact-size is increased by enhancing CD2 expression, strong signaling occurs that is no longer antigen dependent. Our observations suggest that, modulated by the opposing effects of the target cell glycocalyx and small adhesive proteins, the use of microvilli equips T cells with the ability to effect discriminatory receptor signaling.