Nature communications, 2020-01, Vol.11 (1), p.304-304, Article 304
2020
Details
- Autor(en) / Beteiligte
- Titel
- Tunable light and drug induced depletion of target proteins
- Ist Teil von
- Nature communications, 2020-01, Vol.11 (1), p.304-304, Article 304
- Ort / Verlag
- England: Nature Publishing Group
- Erscheinungsjahr
- 2020
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Biological processes in development and disease are controlled by the abundance, localization and modification of cellular proteins. We have developed versatile tools based on recombinant E3 ubiquitin ligases that are controlled by light or drug induced heterodimerization for nanobody or DARPin targeted depletion of endogenous proteins in cells and organisms. We use this rapid, tunable and reversible protein depletion for functional studies of essential proteins like PCNA in DNA repair and to investigate the role of CED-3 in apoptosis during Caenorhabditis elegans development. These independent tools can be combined for spatial and temporal depletion of different sets of proteins, can help to distinguish immediate cellular responses from long-term adaptation effects and can facilitate the exploration of complex networks.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2041-1723eISSN: 2041-1723DOI: 10.1038/s41467-019-14160-8Titel-ID: cdi_doaj_primary_oai_doaj_org_article_e0b9331a777b4a65a1b6e1126958bf30
- Format
- –
- Schlagworte
- Animals, Apoptosis, Biological activity, Caenorhabditis elegans - genetics, Caenorhabditis elegans - metabolism, Caenorhabditis elegans Proteins - drug effects, Caenorhabditis elegans Proteins - genetics, Caenorhabditis elegans Proteins - metabolism, Caenorhabditis elegans Proteins - radiation effects, Caspases - drug effects, Caspases - metabolism, Caspases - radiation effects, Cell Engineering - methods, Control methods, Cytological Techniques, Deoxyribonucleic acid, Depletion, Disease control, DNA, DNA Damage, DNA Ligase ATP, DNA Repair, DNA-Binding Proteins - metabolism, Gene Expression Regulation, Developmental, Genetic engineering, Green Fluorescent Proteins, HeLa Cells, Humans, Lamin Type A - metabolism, Light, Localization, Proliferating cell nuclear antigen, Proliferating Cell Nuclear Antigen - metabolism, Protein engineering, Proteins, Transcription Factors - metabolism, Ubiquitin, Ubiquitin-Protein Ligases - drug effects, Ubiquitin-Protein Ligases - genetics, Ubiquitin-Protein Ligases - metabolism, Ubiquitin-Protein Ligases - radiation effects