Nature communications, 2018-05, Vol.9 (1), p.1933-13, Article 1933
2018
Details
- Autor(en) / Beteiligte
- Titel
- Precise control of SCRaMbLE in synthetic haploid and diploid yeast
- Ist Teil von
- Nature communications, 2018-05, Vol.9 (1), p.1933-13, Article 1933
- Ort / Verlag
- England: Nature Publishing Group
- Erscheinungsjahr
- 2018
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Compatibility between host cells and heterologous pathways is a challenge for constructing organisms with high productivity or gain of function. Designer yeast cells incorporating the Synthetic Chromosome Rearrangement and Modification by LoxP-mediated Evolution (SCRaMbLE) system provide a platform for generating genotype diversity. Here we construct a genetic AND gate to enable precise control of the SCRaMbLE method to generate synthetic haploid and diploid yeast with desired phenotypes. The yield of carotenoids is increased to 1.5-fold by SCRaMbLEing haploid strains and we determine that the deletion of YEL013W is responsible for the increase. Based on the SCRaMbLEing in diploid strains, we develop a strategy called Multiplex SCRaMbLE Iterative Cycling (MuSIC) to increase the production of carotenoids up to 38.8-fold through 5 iterative cycles of SCRaMbLE. This strategy is potentially a powerful tool for increasing the production of bio-based chemicals and for mining deep knowledge.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2041-1723eISSN: 2041-1723DOI: 10.1038/s41467-018-03084-4Titel-ID: cdi_doaj_primary_oai_doaj_org_article_e08f2e5c10a842a095d6c577d5999efa
- Format
- –
- Schlagworte
- Base Sequence, Carotenoids, Carotenoids - biosynthesis, Chromosomes, Fungal - chemistry, Clonal deletion, Clone Cells, Data mining, Gene Deletion, Gene Expression Regulation, Fungal, Genes, Synthetic, Genome, Fungal, Genotypes, Integrases - genetics, Integrases - metabolism, Metabolic Engineering - methods, Metabolic Networks and Pathways - genetics, Phenotype, Phenotypes, Plasmids - chemistry, Plasmids - metabolism, Ploidies, Recombination, Genetic, Saccharomyces cerevisiae - genetics, Saccharomyces cerevisiae - metabolism, Saccharomyces cerevisiae Proteins - genetics, Strains (organisms), Vesicular Transport Proteins - deficiency, Vesicular Transport Proteins - genetics, Yeast