International journal of molecular sciences, 2020-07, Vol.21 (14), p.4838
2020
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- Autor(en) / Beteiligte
- Titel
- Distinct Chemical Changes in Abdominal but Not in Thoracic Aorta upon Atherosclerosis Studied Using Fiber Optic Raman Spectroscopy
- Ist Teil von
- International journal of molecular sciences, 2020-07, Vol.21 (14), p.4838
- Ort / Verlag
- Switzerland: MDPI AG
- Erscheinungsjahr
- 2020
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Fiber optic Raman spectroscopy and Raman microscopy were used to investigate alterations in the aorta wall and the surrounding perivascular adipose tissue (PVAT) in the murine model of atherosclerosis ( mice). Both abdominal and thoracic parts of the aorta were studied to account for the heterogenic chemical composition of aorta and its localization-dependent response in progression of atherosclerosis. The average Raman spectra obtained for both parts of aorta cross sections revealed that the chemical composition of intima-media layers along aorta remains relatively homogeneous while the lipid content in the adventitia layer markedly increases with decreasing distance to PVAT. Moreover, our results demonstrate that the increase of the lipid to protein ratio in the aorta wall correlates directly with the increased unsaturation level of lipids in PVAT and these changes occur only in the abdominal, but not in thoracic, aorta. In summary, distinct pathophysiological response in the aortic vascular wall could be uncovered by fiber optic Raman spectroscopy based on simple parameters detecting chemical contents of lipids in PVAT.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1422-0067, 1661-6596eISSN: 1422-0067DOI: 10.3390/ijms21144838Titel-ID: cdi_doaj_primary_oai_doaj_org_article_df85e68c69e2492ab3061dba294257ef
- Format
- –
- Schlagworte
- Abdomen, Adipose tissue, Adipose Tissue - metabolism, Animal models, Animals, Aorta, Aorta, Abdominal - metabolism, Aorta, Thoracic - metabolism, Apolipoprotein E, Apolipoproteins E - metabolism, Arteriosclerosis, Atherosclerosis, Atherosclerosis - metabolism, Cardiovascular disease, Carotenoids, Chemical composition, Coronary vessels, Cytokines, Endothelium, Fatty acids, fiber optic probe, Fiber optics, Heart surgery, Inflammation, Lipids, Lipids - chemistry, Localization, Male, Mice, Mice, Inbred C57BL, Obesity, Optical fibers, perivascular adipose tissue, Raman spectra, Raman spectroscopy, Receptors, LDL - metabolism, Spectrum analysis, Spectrum Analysis, Raman - methods, thoracic and abdominal aorta, Thorax, vascular inflammation