Details

Autor(en) / Beteiligte

• Riviere-Cazaux, Cecile
• Carlstrom, Lucas P
• Rajani, Karishma
• Munoz-Casabella, Amanda
• Rahman, Masum
• Gharibi-Loron, Ali
• Brown, Desmond A
• Miller, Kai J
• White, Jaclyn J
• Himes, Benjamin T
• Jusue-Torres, Ignacio
• Ikram, Samar
• Ransom, Seth C
• Hirte, Renee
• Oh, Ju-Hee
• Elmquist, William F
• Sarkaria, Jann N
• Vaubel, Rachael A
• Rodriguez, Moses
• Warrington, Arthur E
• Kizilbash, Sani H
• Burns, Terry C

Titel

Blood-brain barrier disruption defines the extracellular metabolome of live human high-grade gliomas

Ist Teil von

• Communications biology, 2023-06, Vol.6 (1), p.653-653, Article 653

Ort / Verlag

England: Nature Publishing Group

Erscheinungsjahr

2023

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• The extracellular microenvironment modulates glioma behaviour. It remains unknown if blood-brain barrier disruption merely reflects or functionally supports glioma aggressiveness. We utilised intra-operative microdialysis to sample the extracellular metabolome of radiographically diverse regions of gliomas and evaluated the global extracellular metabolome via ultra-performance liquid chromatography tandem mass spectrometry. Among 162 named metabolites, guanidinoacetate (GAA) was 126.32x higher in enhancing tumour than in adjacent brain. 48 additional metabolites were 2.05-10.18x more abundant in enhancing tumour than brain. With exception of GAA, and 2-hydroxyglutarate in IDH-mutant gliomas, differences between non-enhancing tumour and brain microdialysate were modest and less consistent. The enhancing, but not the non-enhancing glioma metabolome, was significantly enriched for plasma-associated metabolites largely comprising amino acids and carnitines. Our findings suggest that metabolite diffusion through a disrupted blood-brain barrier may largely define the enhancing extracellular glioma metabolome. Future studies will determine how the altered extracellular metabolome impacts glioma behaviour.