Details

Autor(en) / Beteiligte

• Faramayuda, Fahrauk
• Syam, Akhirul Kahfi
• Mariani, Totik Sri
• Elfahmi
• Sukrasno

Titel

Effect of Media Variation on the Induction and Phytochemical Profile of Callus in Two Varieties of Cat's Whiskers (Orthosiphon aristatus Blume Miq)

Ist Teil von

• Hayati : journal of biosciences, 2022-09, Vol.30 (1), p.159-170

Ort / Verlag

Bogor Agricultural University

Erscheinungsjahr

2022

Link zum Volltext

Quelle

EZB Free E-Journals

Beschreibungen/Notizen

• The levels of rosmarinic acid and sinensetin in purple and white-purple varieties of Orthosiphon aristatus, cat's whiskers, can be increased using modified in vitro culture. This work focused on callus induction of the purple and white-purple variety of cat's whiskers grown on Gamborg (B5) and CHU (N6) with the addition of growth regulators 2,4-dichlorophenoxyacetis acid. Our observation suggested that the callus could grow within three weeks and produce rosmarinic acid and sinensetin. The level of sinensetin from various extraction methods is relatively low; in contrast, the rosmarinic acid from purple callus was detected at about 5% w/w, while the white-purple variety was around 2% w/w. The results of this study also provided new information on the basic media other than MS that can grow cat's whiskers callus while producing active compounds.