Details

Autor(en) / Beteiligte

• Jenett, Arnim
• Rubin, Gerald M.
• Ngo, Teri-T.B.
• Shepherd, David
• Murphy, Christine
• Dionne, Heather
• Pfeiffer, Barret D.
• Cavallaro, Amanda
• Hall, Donald
• Jeter, Jennifer
• Iyer, Nirmala
• Fetter, Dona
• Hausenfluck, Joanna H.
• Peng, Hanchuan
• Trautman, Eric T.
• Svirskas, Robert R.
• Myers, Eugene W.
• Iwinski, Zbigniew R.
• Aso, Yoshinori
• DePasquale, Gina M.
• Enos, Adrianne
• Hulamm, Phuson
• Lam, Shing Chun Benny
• Li, Hsing-Hsi
• Laverty, Todd R.
• Long, Fuhui
• Qu, Lei
• Murphy, Sean D.
• Rokicki, Konrad
• Safford, Todd
• Shaw, Kshiti
• Simpson, Julie H.
• Sowell, Allison
• Tae, Susana
• Yu, Yang
• Zugates, Christopher T.

Titel

A GAL4-Driver Line Resource for Drosophila Neurobiology

Ist Teil von

• Cell reports (Cambridge), 2012-10, Vol.2 (4), p.991-1001

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2012

Quelle

MEDLINE

Beschreibungen/Notizen

• We established a collection of 7,000 transgenic lines of Drosophila melanogaster. Expression of GAL4 in each line is controlled by a different, defined fragment of genomic DNA that serves as a transcriptional enhancer. We used confocal microscopy of dissected nervous systems to determine the expression patterns driven by each fragment in the adult brain and ventral nerve cord. We present image data on 6,650 lines. Using both manual and machine-assisted annotation, we describe the expression patterns in the most useful lines. We illustrate the utility of these data for identifying novel neuronal cell types, revealing brain asymmetry, and describing the nature and extent of neuronal shape stereotypy. The GAL4 lines allow expression of exogenous genes in distinct, small subsets of the adult nervous system. The set of DNA fragments, each driving a documented expression pattern, will facilitate the generation of additional constructs for manipulating neuronal function. [Display omitted] ► Seven thousand GAL4 lines are made using defined enhancer fragments and integration site ► Expression patterns of 6,650 lines are imaged in the adult brain and VNC ► The fly brain displays limited left-right asymmetry ► GAL4 lines and high-resolution image data are available to the community Rubin and colleagues have established a collection of 7,000 GAL4 driver lines in Drosophila. A different, defined fragment of genomic DNA serves as a transcriptional enhancer to control GAL4 expression in each line. The authors describe the expression patterns driven by each fragment in the adult brain and ventral nerve cord and illustrate the utility of these data in identifying novel neuronal cell types, revealing brain asymmetry, and describing the nature and extent of neuronal shape stereotypy.