BMC research notes, 2021-05, Vol.14 (1), p.212-212, Article 212
2021
Details
- Autor(en) / Beteiligte
- Titel
- Applicability of human-specific STR systems, GlobalFiler™ PCR Amplification Kit, Investigator 24plex QS Kit, and PowerPlex® Fusion 6C in chimpanzee (Pan troglodytes)
- Ist Teil von
- BMC research notes, 2021-05, Vol.14 (1), p.212-212, Article 212
- Ort / Verlag
- England: BioMed Central Ltd
- Erscheinungsjahr
- 2021
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Human identification systems based on STRs are widely used in human population genetics and forensic analysis. This study aimed to validate the cross-reactivity of three widely known human-specific STR identification systems i.e. GlobalFiler™ PCR Amplification Kit, Investigator 24plex QS Kit, and PowerPlex® Fusion 6C in chimpanzee. The present study revealed the successful amplification of 18 loci using GlobalFiler™ PCR Amplification Kit, 18 loci using Investigator 24plex QS Kit, and 20 loci using PowerPlex® Fusion 6C system. The marker Amelogenin (AMEL) showed differential allele size between male and female revealing the gender identity of chimpanzees and thus validates their application concerning forensic examination, population estimation, and genetic analysis.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1756-0500eISSN: 1756-0500DOI: 10.1186/s13104-021-05632-6Titel-ID: cdi_doaj_primary_oai_doaj_org_article_daddb22ab5ae4575ba2a2f48926ea554
- Format
- –
- Schlagworte
- Animals, Chimpanzee, Cross-reactivity, DNA Fingerprinting, DNA testing, Female, Forensic science, Gender, Gender Identity, Genetic analysis, Genetic diversity, GlobalFiler™ PCR amplification kit, Human identification, Humans, Identification systems, Investigator 24plex QS kit, Male, Methods, Microsatellite Repeats - genetics, Monkeys & apes, Pan troglodytes - genetics, Polymerase Chain Reaction, Population genetics, PowerPlex® fusion 6C system, Primates, Research Note, Sample size, Short tandem repeats