Details

Autor(en) / Beteiligte

• Giang, Ngo Truong
• Tong, Hoang Van
• Nghia, Trinh Huu
• Hung, Hoang Vu
• Anh, Do Tuan
• Nam, Le Van
• Mao, Can Van
• Giang, Nguyen Truong
• Thanh, Le Dinh
• Son, Ho Anh
• Velavan, Thirumalaisamy P.
• Do, Quyet
• Toan, Nguyen Linh

Titel

Association of FCN2 polymorphisms and Ficolin-2 levels with dengue fever in Vietnamese patients

Ist Teil von

• International journal of infectious diseases, 2020-06, Vol.95, p.253-261

Ort / Verlag

Canada: Elsevier Ltd

Erscheinungsjahr

2020

Link zum Volltext

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• • FCN2 genetic polymorphisms are associated with dengue fever in the Vietnamese population.• Ficolin-2 levels were significantly modulated during the progression of dengue fever• Ficolin-2 levels were correlated with AST levels and with platelet counts.• Ficolin-2 may play an important role in the pathogenesis of DENV infection. The human ficolin-2, encoded by FCN2, recognizes pathogen-associated acetylated residues on their cell surfaces and activates the lectin complement cascade. This study aimed to investigate the contribution of human ficolin-2 and the functional FCN2 genetic variants in dengue virus (DENV) infection and in clinical progression. FCN2 genetic polymorphisms in the promoter, intron 7 and exon 8 were genotyped in 279 patients with dengue fever and in 200 healthy controls by direct Sanger sequencing. The ficolin-2 levels were measured in serum samples by ELISA and correlated with clinical data. The frequencies of +6031GG, +6220GG and +6424TT genotypes were significantly higher in dengue patients compared to healthy controls indicating an increased risk of dengue fever. The SNPs rs11103563 (+6031A/G), rs7872508 (+6220 T/G), and rs7851696 (+6424G/T) significantly regulated ficolin-2 levels in dengue patients (P < 0.0001). Ficolin-2 levels were increased in patients with dengue and Dengue with Warning Signs (DWS) compared to healthy controls (P < 0.0001 and P = 0.038, respectively). Ficolin-2 levels were significantly increased after 10–14 days of admission in both dengue and DWS patients and then slightly decreased after three weeks of discharge, indicating that ficolin-2 levels were modulated during the progression of dengue fever. In addition, ficolin-2 levels were negatively correlated with AST levels and positively correlated with platelet counts. FCN2 polymorphisms are associated with dengue fever in the Vietnamese population. Ficolin-2 levels are modulated during the progression of dengue fever and correlated with clinical parameters and thus may play a possible role in the pathogenesis of DENV infection.