Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
Amino acid substitutions in a polygalacturonase inhibiting protein (OsPGIP2) increases sheath blight resistance in rice
Ist Teil von
Rice (New York, N.Y.), 2019-07, Vol.12 (1), p.56-12, Article 56
Ort / Verlag
New York: Springer US
Erscheinungsjahr
2019
Quelle
Alma/SFX Local Collection
Beschreibungen/Notizen
Background
An economic strategy to control plant disease is to improve plant defense to pathogens by deploying resistance genes. Plant polygalacturonase inhibiting proteins (PGIPs) have a vital role in plant defense against phytopathogenic fungi by inhibiting fungal polygalacturonase (PG) activity. We previously reported that rice PGIP1 (OsPGIP1) inhibits PG activity in
Rhizoctonia solani
, the causal agent of rice sheath blight (SB), and is involved in regulating resistance to SB.
Result
Here, we report that OsPGIP2, the protein ortholog of OsPGIP1, does not possess PGIP activity; however, a few amino acid substitutions in a derivative of OsPGIP2, of which we provide support for L233F being the causative mutation, appear to impart OsPGIP2 with PG inhibition capability. Furthermore, the overexpression of mutated
OsPGIP2
L233F
in rice significantly increased the resistance of transgenic lines and decreased SB disease rating scores.
OsPGIP2
L233F
transgenic lines displayed an increased ability to reduce the tissue degradation caused by
R. solani
PGs as compared to control plants. Rice plants overexpressing
OsPGIP2
L233F
showed no difference in agronomic traits and grain yield as compared to controls, thus demonstrating its potential use in rice breeding programs.
Conclusions
In summary, our results provide a new target gene for breeding SB resistance through genome-editing or natural allele mining.