Details

Autor(en) / Beteiligte

• McCracken, Michael K.
• Kuklis, Caitlin H.
• Kannadka, Chandrika B.
• Barvir, David A.
• Sanborn, Mark A.
• Waickman, Adam T.
• Siegfried, Hayden C.
• Victor, Kaitlin A.
• Hatch, Kristin L.
• De La Barrera, Rafael
• Walls, Shannon D.
• Rutvisuttinunt, Wiriya
• Currier, Jeffrey R.
• Friberg, Heather
• Jarman, Richard G.
• Gromowski, Gregory D.

Titel

Enhanced dengue vaccine virus replication and neutralizing antibody responses in immune primed rhesus macaques

Ist Teil von

• npj vaccines, 2021-05, Vol.6 (1), p.77-77, Article 77

Ort / Verlag

London: Nature Publishing Group

Erscheinungsjahr

2021

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Abstract Antibody-dependent enhancement (ADE) is suspected to influence dengue virus (DENV) infection, but the role ADE plays in vaccination strategies incorporating live attenuated virus components is less clear. Using a heterologous prime-boost strategy in rhesus macaques, we examine the effect of priming with DENV purified inactivated vaccines (PIVs) on a tetravalent live attenuated vaccine (LAV). Sera exhibited low-level neutralizing antibodies (NAb) post PIV priming, yet moderate to high in vitro ADE activity. Following LAV administration, the PIV primed groups exhibited DENV-2 LAV peak viremias up to 1,176-fold higher than the mock primed group, and peak viremia correlated with in vitro ADE. Furthermore, PIV primed groups had more balanced and higher DENV-1–4 NAb seroconversion and titers than the mock primed group following LAV administration. These results have implications for the development of effective DENV vaccine prime-boost strategies and for our understanding of the role played by ADE in modulating DENV replication.