Details

Autor(en) / Beteiligte

• Ando, Jun
• Murai, Kazue
• Mori, Makiko
• Michiyuki, Tomoe
• Iida, Tatsuya
• Makino, Asami
• Shinoda, Hajime
• Watanabe, Rikiya

Titel

Exploring fluoropolymers for fabrication of femtoliter chamber arrays used in digital bioanalysis

Ist Teil von

• Scientific reports, 2024-05, Vol.14 (1), p.11442-11442, Article 11442

Ort / Verlag

England: Nature Publishing Group

Erscheinungsjahr

2024

Quelle

Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals

Beschreibungen/Notizen

• The global supply of fluoropolymers and fluorinated solvents is decreasing due to environmental concerns regarding polyfluoroalkyl substances. CYTOP has been used for decades primarily as a component of a femtoliter chamber array for digital bioanalysis; however, its supply has recently become scarce, increasing the urgency of fabricating a femtoliter chamber array using alternative materials. In this study, we investigated the feasibility of fabricating a femtoliter chamber array using four types of fluoropolymers in stable supply as candidate substitutes and verified their applicability for digital bioanalysis. Among these candidates, Fluorine Sealant emerged as a viable option for fabricating femtoliter chamber arrays using a conventional photolithography process. To validate its efficacy, we performed various digital bioanalysis using FP-A-based chamber arrays with model enzymes such as CRISPR-Cas, horseradish peroxidase, and β-galactosidase. The results demonstrated the similar performance to that of CYTOP, highlighting the broader utility of FP-A in digital bioanalysis. Our findings underscore the potential of FP-A to enhance the versatility of digital bioanalysis and foster the ongoing advancement of innovative diagnostic technologies.