Details

Autor(en) / Beteiligte

• Park, Young Eun
• Penumarthy, Rushi
• Sun, Paul P
• Kang, Caroline Y
• Morel-Kopp, Marie-Christine
• Downing, Jonathan
• Green, Taryn N
• Immanuel, Tracey
• Ward, Christopher M
• Young, Deborah
• During, Matthew J
• Barber, P Alan
• Kalev-Zylinska, Maggie L

Titel

Platelet-Reactive Antibodies in Patients after Ischaemic Stroke-An Epiphenomenon or a Natural Protective Mechanism

Ist Teil von

• International journal of molecular sciences, 2020-11, Vol.21 (21), p.8398

Ort / Verlag

Switzerland: MDPI

Erscheinungsjahr

2020

Quelle

MEDLINE

Beschreibungen/Notizen

• Ischaemic brain damage induces autoimmune responses, including the production of autoantibodies with potential neuroprotective effects. Platelets share unexplained similarities with neurons, and the formation of anti-platelet antibodies has been documented in neurological disorders. The aim of this study was to investigate the presence of anti-platelet antibodies in the peripheral blood of patients after ischaemic stroke and determine any clinical correlations. Using a flow cytometry-based platelet immunofluorescence method, we detected platelet-reactive antibodies in 15 of 48 (31%) stroke patients and two of 50 (4%) controls ( < 0.001). Western blotting revealed heterogeneous reactivities with platelet proteins, some of which overlapped with brain proteins. Stroke patients who carried anti-platelet antibodies presented with larger infarcts and more severe neurological dysfunction, which manifested as higher scores on the National Institutes of Health Stroke Scale (NIHSS; 0.009), but they had a greater recovery in the NIHSS by the time of hospital discharge (day 7 ± 2) compared with antibody-negative patients ( = 0.043). Antibodies from stroke sera reacted more strongly with activated platelets ( = 0.031) and inhibited platelet aggregation by up to 30.1 ± 2.8% ( 0.001), suggesting the potential to interfere with thrombus formation. In conclusion, platelet-reactive antibodies can be found in patients soon after ischaemic stroke and correlate with better short-term outcomes, suggesting a potential novel mechanism limiting thrombosis.