Details
- Autor(en) / Beteiligte
- Titel
- Structure of Vps4 with circular peptides and implications for translocation of two polypeptide chains by AAA+ ATPases
- Ist Teil von
- eLife, 2019-06, Vol.8
- Ort / Verlag
- England: eLife Sciences Publications, Ltd
- Erscheinungsjahr
- 2019
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Many AAA+ ATPases form hexamers that unfold protein substrates by translocating them through their central pore. Multiple structures have shown how a helical assembly of subunits binds a single strand of substrate, and indicate that translocation results from the ATP-driven movement of subunits from one end of the helical assembly to the other end. To understand how more complex substrates are bound and translocated, we demonstrated that linear and cyclic versions of peptides bind to the AAA+ ATPase Vps4 with similar affinities, and determined cryo-EM structures of cyclic peptide complexes. The peptides bind in a hairpin conformation, with one primary strand equivalent to the single chain peptide ligands, while the second strand returns through the translocation pore without making intimate contacts with Vps4. These observations indicate a general mechanism by which AAA+ ATPases may translocate a variety of substrates that include extended chains, hairpins, and crosslinked polypeptide chains.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2050-084XeISSN: 2050-084XDOI: 10.7554/eLife.44071Titel-ID: cdi_doaj_primary_oai_doaj_org_article_d6a79491d33f44f68941cb6081b14f95
- Format
- –
- Schlagworte
- AAA+ ATPase, Adenosine Triphosphatases - chemistry, Adenosine Triphosphatases - genetics, Adenosine Triphosphatases - metabolism, Amino Acid Sequence, ATPases Associated with Diverse Cellular Activities - chemistry, ATPases Associated with Diverse Cellular Activities - genetics, ATPases Associated with Diverse Cellular Activities - metabolism, Binding, Competitive, Biochemistry and Chemical Biology, cryo-EM, Cryoelectron Microscopy, Endosomal Sorting Complexes Required for Transport - chemistry, Endosomal Sorting Complexes Required for Transport - genetics, Endosomal Sorting Complexes Required for Transport - metabolism, Endosomes, Models, Molecular, Peptides - chemistry, Peptides - metabolism, Peptides, Cyclic - chemistry, Peptides, Cyclic - metabolism, Protein Binding, Protein Conformation, Protein Multimerization, protein structure, Saccharomyces cerevisiae - genetics, Saccharomyces cerevisiae - metabolism, Saccharomyces cerevisiae Proteins - chemistry, Saccharomyces cerevisiae Proteins - genetics, Saccharomyces cerevisiae Proteins - metabolism, Structural Biology and Molecular Biophysics