BMC microbiology, 2017-07, Vol.17 (1), p.153-153, Article 153
2017
Details
- Autor(en) / Beteiligte
- Titel
- Hypothetical protein Cpn0423 triggers NOD2 activation and contributes to Chlamydia pneumoniae-mediated inflammation
- Ist Teil von
- BMC microbiology, 2017-07, Vol.17 (1), p.153-153, Article 153
- Ort / Verlag
- England: BioMed Central Ltd
- Erscheinungsjahr
- 2017
- Link zum Volltext
- Quelle
- SpringerLink (Online service)
- Beschreibungen/Notizen
- Chlamydia pneumoniae (C. pneumoniae) is pathogenic to humans, by causing pulmonary inflammation or bronchitis in both adolescents and young adults. However, the molecular signals linking C. pneumoniae components to inflammation remain elusive. This study was to investigate the effect of Chlamydia-specific Cpn0423 of C. pneumoniae on C. pneumoniae-mediated inflammation. Cpn0423 was detected outside of C. pneumoniae inclusions, which induced production of several cytokines including macrophage inflammatory protein-2 (MIP-2) and interleukins (ILs). Production of the Cpn0423-induced cytokines was markedly reduced in cells pretreated with NOD2-siRNA, but not with negative control oligonucleotides. Mice treated with Cpn0423 through intranasal administration exhibited pulmonary inflammation as evidenced by infiltration of inflammatory cells, increased inflammatory scores in the lung histology, recruitment of neutrophils and increased cytokines levels in the BALF. Cpn0423 could be sensed by NOD2, which was identified as an essential element in a pathway contributing to the development of C. pneumoniae -mediated inflammation.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1471-2180eISSN: 1471-2180DOI: 10.1186/s12866-017-1062-yTitel-ID: cdi_doaj_primary_oai_doaj_org_article_d50912b7b4dc42da931aa2f873921aaf
- Format
- –
- Schlagworte
- Adolescents, Adults, Animals, Bacteria, Bacterial infections, Bacterial Proteins - genetics, Bacterial Proteins - immunology, Bronchitis, Cardiovascular disease, Chemokine CXCL2 - genetics, Chemokine CXCL2 - immunology, Chlamydia, Chlamydia pneumoniae, Chlamydophila Infections - genetics, Chlamydophila Infections - immunology, Chlamydophila Infections - microbiology, Chlamydophila pneumoniae - genetics, Chlamydophila pneumoniae - immunology, Chronic illnesses, Cpn0423, Cytokines, Cytoplasm, Health aspects, Heat shock proteins, Histology, Humans, Immune response, Infections, Infiltration, Inflammation, Inflammation Mediators - immunology, Inflammatory, Inflammatory diseases, Interleukins, Interleukins - genetics, Interleukins - immunology, Intranasal administration, Kinases, Leukocytes (neutrophilic), Lung - immunology, Lungs, Macrophage inflammatory protein, Macrophages, Male, Mice, Mice, Inbred C57BL, NOD2, NOD2 protein, Nod2 Signaling Adaptor Protein - genetics, Nod2 Signaling Adaptor Protein - immunology, Observations, Oligonucleotides, Pathogens, Pneumonia, Pneumonia, Bacterial - genetics, Pneumonia, Bacterial - immunology, Pneumonia, Bacterial - microbiology, Recruitment, Sepsis, Sexually transmitted diseases, siRNA, STD, Young adults