Details

Autor(en) / Beteiligte

• Ding, Chi Ying Gary
• Pang, Li Mei
• Liang, Zhao-Xun
• Goh, Kau Kiat Kelvin
• Glukhov, Evgenia
• Gerwick, William H
• Tan, Lik Tong

Titel

MS/MS-Based Molecular Networking Approach for the Detection of Aplysiatoxin-Related Compounds in Environmental Marine Cyanobacteria

Ist Teil von

• Marine drugs, 2018-12, Vol.16 (12), p.505

Ort / Verlag

Switzerland: MDPI AG

Erscheinungsjahr

2018

Link zum Volltext

Quelle

EZB Free E-Journals

Beschreibungen/Notizen

• Certain strains of cyanobacteria produce a wide array of cyanotoxins, such as microcystins, lyngbyatoxins and aplysiatoxins, that are associated with public health issues. In this pilot study, an approach combining LC-MS/MS and molecular networking was employed as a rapid analytical method to detect aplysiatoxins present in four environmental marine cyanobacterial samples collected from intertidal areas in Singapore. Based on 16S-ITS rRNA gene sequences, these filamentous cyanobacterial samples collected from Pulau Hantu were determined as , sp. PAB-2 and sp. PNG05-4. Organic extracts were prepared and analyzed on LC-HRMS/MS and Global Natural Product Social Molecular Networking (GNPS) for the presence of aplysiatoxin-related molecules. From the molecular networking, six known compounds, debromoaplysiatoxin ( ), anhydrodebromoaplysiatoxin ( ), 3-methoxydebromoaplysiatoxin ( ), aplysiatoxin ( ), oscillatoxin A ( ) and 31-noroscillatoxin B ( ), as well as potential new analogues, were detected in these samples. In addition, differences and similarities in molecular networking clusters related to the aplysiatoxin molecular family were observed in extracts of collected from two different locations and from different cyanobacterial species found at Pulau Hantu, respectively.