Details

Autor(en) / Beteiligte

• Zelman-Femiak, Monika
• Wang, Kun
• Gromova, Kira V
• Knaus, Petra
• Harms, Gregory S

Titel

Covalent quantum dot receptor linkage via the acyl carrier protein for single-molecule tracking, internalization, and trafficking studies

Ist Teil von

• BioTechniques, 2010-08, Vol.49 (2), p.574-579

Ort / Verlag

Natick, MA: Future Science Ltd

Erscheinungsjahr

2010

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Here we describe a labeling technique for the covalent linkage of quantum dots to transmembrane receptors for single-molecule tracking. Our method combines the acyl carrier protein (ACP) technique with coenzyme A (CoA)-functionalized quantum dots to covalently attach quantum dots to ACP fusions of receptor proteins. The advantages of this approach include: ( ) the use of a smaller attachment linker than in many other quantum dot-labeling systems; ( ) the ability to achieve a reliable 1:1 fluorophore-to-receptor labeling stoichiometry; ( ) the specificity of the method; and ( ) the covalent nature of the quantum dot linkage. We demonstrate the general suitability of this technique in single-molecule tracking, internalization, and trafficking studies by imaging two different transmembrane receptors in living cells.