Genome Biology, 2019-01, Vol.20 (1), p.19-19, Article 19
2019
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- Autor(en) / Beteiligte
- Titel
- Degradation of endogenous proteins and generation of a null-like phenotype in zebrafish using Trim-Away technology
- Ist Teil von
- Genome Biology, 2019-01, Vol.20 (1), p.19-19, Article 19
- Ort / Verlag
- England: BioMed Central
- Erscheinungsjahr
- 2019
- Quelle
- SpringerLink
- Beschreibungen/Notizen
- Trim-Away is a recent technique to rapidly deplete a protein from any cell type. Guided by antibodies, TRIM21 selects proteins for destruction. However, the applicability of this method in model organisms has not been investigated. Here, we show that Trim-Away can degrade proteins in zebrafish embryos. Trim-Away depletes proteins faster than morpholinos, which enables analysis of protein function during early embryogenesis. Furthermore, Trim-Away can be applied to evaluate the role of maternally contributed proteins in zebrafish embryos. Our findings indicate that Trim-Away is a powerful tool to perform functional analysis of proteins during zebrafish development.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1474-760X, 1474-7596eISSN: 1474-760XDOI: 10.1186/s13059-019-1624-4Titel-ID: cdi_doaj_primary_oai_doaj_org_article_ce389ae3ce3b484e9e8d1498c072704c
- Format
- –
- Schlagworte
- Animals, Animals, Genetically Modified, Biotechnology - methods, Danio rerio, Defects, Embryo, Nonmammalian - metabolism, Embryogenesis, Gene expression, Genomes, Immunoglobulins, Knockdown, Morphology, Phenotypes, Protein degradation, Proteins, Proteolysis, Ribonucleoproteins - metabolism, Short Report, Trim-away, TRIM21, Zebrafish