International journal of molecular sciences, 2023-02, Vol.24 (4), p.3467
2023
Details
- Autor(en) / Beteiligte
- Titel
- Long-Term Ingestion of Sicilian Black Bee Chestnut Honey and/or D-Limonene Counteracts Brain Damage Induced by High Fat-Diet in Obese Mice
- Ist Teil von
- International journal of molecular sciences, 2023-02, Vol.24 (4), p.3467
- Ort / Verlag
- Switzerland: MDPI AG
- Erscheinungsjahr
- 2023
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Obesity is linked to neurodegeneration, which is mainly caused by inflammation and oxidative stress. We analyzed whether the long-term intake of honey and/or D-limonene, which are known for their antioxidant and anti-inflammatory actions, when ingested separately or in combination, can counteract the neurodegeneration occurring in high fat diet (HFD)-induced obesity. After 10 weeks of HFD, mice were divided into: HFD-, HFD + honey (HFD-H)-, HFD + D-limonene (HFD-L)-, HFD + honey + D-limonene (HFD-H + L)-fed groups, for another 10 weeks. Another group was fed a standard diet (STD). We analyzed the brain neurodegeneration, inflammation, oxidative stress, and gene expression of Alzheimer's disease (AD) markers. The HFD animals showed higher neuronal apoptosis, upregulation of pro-apoptotic genes Fas-L, Bim P27 and downregulation of anti-apoptotic factors BDNF and BCL2; increased gene expression of the pro-inflammatory IL-1β, IL-6 and TNF-α and elevated oxidative stress markers COX-2, iNOS, ROS and nitrite. The honey and D-limonene intake counteracted these alterations; however, they did so in a stronger manner when in combination. Genes involved in amyloid plaque processing (APP and TAU), synaptic function (Ache) and AD-related hyperphosphorylation were higher in HFD brains, and significantly downregulated in HFD-H, HFD-L and HFD-H + L. These results suggest that honey and limonene ingestion counteract obesity-related neurodegeneration and that joint consumption is more efficacious than a single administration.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1422-0067, 1661-6596eISSN: 1422-0067DOI: 10.3390/ijms24043467Titel-ID: cdi_doaj_primary_oai_doaj_org_article_cad8cce0702f4ec383aedca8e0dcccfd
- Format
- –
- Schlagworte
- Alzheimer's disease, Amyloid, Analysis, Animals, Apoptosis, Bees, BIM protein, Brain - metabolism, Brain damage, Brain Injuries - metabolism, Brain injury, Brain-derived neurotrophic factor, D-limonene, Diet, Diet, High-Fat, Eating, Gene expression, Genes, High fat diet, Honey, Inflammation, Inflammation - metabolism, Ingestion, Interleukin 6, Limonene, Mice, Mice, Inbred C57BL, Mice, Obese, Neurodegeneration, neuronal damage, Neurons, Nitric-oxide synthase, Obesity, Obesity - metabolism, Oils & fats, Oxidative stress, Phosphorylation, Tau protein, Tumor necrosis factor-α