Details

Autor(en) / Beteiligte

• Richter, Anne
• Blei, Felix
• Hu, Guohai
• Schwitalla, Jan W.
• Lozano-Andrade, Carlos N.
• Xie, Jiyu
• Jarmusch, Scott A.
• Wibowo, Mario
• Kjeldgaard, Bodil
• Surabhi, Surabhi
• Xu, Xinming
• Jautzus, Theresa
• Phippen, Christopher B. W.
• Tyc, Olaf
• Arentshorst, Mark
• Wang, Yue
• Garbeva, Paolina
• Larsen, Thomas Ostenfeld
• Ram, Arthur F. J.
• van den Hondel, Cees A. M.
• Maróti, Gergely
• Kovács, Ákos T.

Titel

Enhanced surface colonisation and competition during bacterial adaptation to a fungus

Ist Teil von

• Nature communications, 2024-05, Vol.15 (1), p.4486-4486, Article 4486

Ort / Verlag

London: Nature Publishing Group UK

Erscheinungsjahr

2024

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Bacterial-fungal interactions influence microbial community performance of most ecosystems and elicit specific microbial behaviours, including stimulating specialised metabolite production. Here, we use a co-culture experimental evolution approach to investigate bacterial adaptation to the presence of a fungus, using a simple model of bacterial-fungal interactions encompassing the bacterium Bacillus subtilis and the fungus Aspergillus niger . We find in one evolving population that B. subtilis was selected for enhanced production of the lipopeptide surfactin and accelerated surface spreading ability, leading to inhibition of fungal expansion and acidification of the environment. These phenotypes were explained by specific mutations in the DegS-DegU two-component system. In the presence of surfactin, fungal hyphae exhibited bulging cells with delocalised secretory vesicles possibly provoking an RlmA-dependent cell wall stress. Thus, our results indicate that the presence of the fungus selects for increased surfactin production, which inhibits fungal growth and facilitates the competitive success of the bacterium. Bacterial-fungal interactions can stimulate the production of specialised microbial metabolites. Here, Richter et al. use co-culture experimental evolution to show that the presence of a fungus selects for increased surfactin production in the bacterium Bacillus subtilis , which inhibits fungal growth and facilitates the competitive success of the bacterium.