Details

Autor(en) / Beteiligte

• Iosue, Christine L
• Gulotta, Anthony P
• Selhorst, Kathleen B
• Mody, Alison C
• Barbour, Kristin M
• Marcotte, Meredith J
• Bui, Lilian N
• Leone, Sarah G
• Lang, Emma C
• Hughes, Genevieve H
• Wykoff, Dennis D

Titel

A Novel cis Element Achieves the Same Solution as an Ancestral cis Element During Thiamine Starvation in Candida glabrata

Ist Teil von

• G3 : genes - genomes - genetics, 2020-01, Vol.10 (1), p.321-331

Ort / Verlag

United States: Genetics Society of America

Erscheinungsjahr

2020

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Regulatory networks often converge on very similar sequences to drive transcriptional programs due to constraints on what transcription factors are present. To determine the role of constraint loss on element evolution, we examined the recent appearance of a thiamine starvation regulated promoter in This species lacks the ancestral transcription factor Thi2, but still has the transcription factor Pdc2, which regulates thiamine starvation genes, allowing us to determine the effect of constraint change on a new promoter. We identified two different elements in - one present in the evolutionarily recent gene called , and the other element present in the other thiamine (THI) regulated genes. Reciprocal swaps of the elements and incorporation of the Thi2 transcription factor-binding site into these promoters demonstrate that the two elements are functionally different from one another. Thus, this loss of an imposed constraint on promoter function has generated a novel sequence, suggesting that loss of constraints can generate a non-convergent pathway with the same output.