Details

Autor(en) / Beteiligte

• Lin, Chun Ting
• Ting, Ruei-Teng
• Ou, Yang-Hsuan
• Shao, Tzu-Ling
• Lee, Ming-Chia

Titel

Protein degradation of Lsd1 is mediated by Bre1 yet opposed by Lsd1-interacting lncRNAs during fly follicle development

Ist Teil von

• iScience, 2024-05, Vol.27 (5), p.109683-109683, Article 109683

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2024

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Tissue development, homeostasis, and repair all require efficient progenitor expansion. Lysine-specific demethylase 1 (Lsd1) maintains plastic epigenetic states to promote progenitor proliferation while overexpressed Lsd1 protein causes oncogenic gene expression in cancer cells. However, the precise regulation of Lsd1 protein expression at the molecular level to drive progenitor differentiation remains unclear. Here, using Drosophila melanogaster oogenesis as our experimental system, we discovered molecular machineries that modify Lsd1 protein stability in vivo. Through genetic and biochemical analyses, an E3 ubiquitin ligase, Bre1, was identified as required for follicle progenitor differentiation, likely by mediating Lsd1 protein degradation. Interestingly, specific Lsd1-interacting long non-coding RNAs (LINRs) were found to antagonize Bre1-mediated Lsd1 protein degradation. The intricate interplay discovered among the Lsd1 complex, LINRs and Bre1 provides insight into how Lsd1 protein stability is fine-tuned to underlie progenitor differentiation in vivo. [Display omitted] •Lsd1 protein expression is tuned post-translationally during fly follicle development•Bre1 is an E3 ligase that mediates Lsd1 protein degradation•Bre1 ubiquitinates Lsd1 to underlie follicle progenitor differentiation•Bre1-mediated Lsd1 degradation is opposed by Lsd1-interacting lncRNAs Biochemistry; Molecular mechanism of gene regulation; Developmental biology