International journal of molecular sciences, 2018-08, Vol.19 (9), p.2535
2018
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- A Cell Junctional Protein Network Associated with Connexin-26
- Ist Teil von
- International journal of molecular sciences, 2018-08, Vol.19 (9), p.2535
- Ort / Verlag
- Switzerland: MDPI AG
- Erscheinungsjahr
- 2018
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- mutations are the leading cause of non-syndromic inherited hearing loss. encodes connexin-26 (CX26), which is a connexin (CX) family protein expressed in cochlea, skin, liver, and brain, displaying short cytoplasmic N-termini and C-termini. We searched for CX26 C-terminus binding partners by affinity capture and identified 12 unique proteins associated with cell junctions or cytoskeleton (CGN, DAAM1, FLNB, GAPDH, HOMER2, MAP7, MAPRE2 (EB2), JUP, PTK2B, RAI14, TJP1, and VCL) by using mass spectrometry. We show that, similar to other CX family members, CX26 co-fractionates with TJP1, VCL, and EB2 (EB1 paralogue) as well as the membrane-associated protein ASS1. The adaptor protein CGN (cingulin) co-immuno-precipitates with CX26, ASS1, and TJP1. In addition, CGN co-immunoprecipitation with CX30, CX31, and CX43 indicates that CX association is independent on the CX C-terminus length or sequence. CX26, CGN, FLNB, and DAMM1 were shown to distribute to the organ of Corti and hepatocyte plasma membrane. In the mouse liver, CX26 and TJP1 co-localized at the plasma membrane. In conclusion, CX26 associates with components of other membrane junctions that integrate with the cytoskeleton.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1422-0067, 1661-6596eISSN: 1422-0067DOI: 10.3390/ijms19092535Titel-ID: cdi_doaj_primary_oai_doaj_org_article_c64ee5ea64dd4f5cab4fddc08a37f2ac
- Format
- –
- Schlagworte
- Amino Acid Sequence, Amino acids, Animals, Argininosuccinate Synthase - genetics, Argininosuccinate Synthase - metabolism, Bioinformatics, Biology, C-Terminus, Candidates, Cell junctions, CGN, Cochlea, Communication, connexin, connexin 26, Connexin 26 - genetics, Connexin 26 - metabolism, Connexin 43, Connexins - genetics, Connexins - metabolism, Cytoskeleton, Cytoskeleton - metabolism, DAAM1, deafness, FLNB, Gene expression, Genetics, Genomes, GJB2 gene, Glyceraldehyde-3-phosphate dehydrogenase, Hearing loss, Immunoprecipitation, Informatics, Intercellular Junctions - metabolism, Liver, Mass spectrometry, Mass spectroscopy, Membrane junctions, Membrane proteins, Mice, Inbred BALB C, Mice, Inbred C57BL, Mutation, Organ of Corti, Organ of Corti - metabolism, Peptides, Precipitates, Protein Binding, Protein Interaction Maps, protein-protein interaction, Proteins, Scientific imaging, Sequence Homology, Amino Acid, Skin, TJP1, Zonula Occludens-1 Protein - genetics, Zonula Occludens-1 Protein - metabolism