International journal of molecular sciences, 2020-03, Vol.21 (6), p.2165
2020
Details
- Autor(en) / Beteiligte
- Titel
- Degeneration of Lumbar Intervertebral Discs: Characterization of Anulus Fibrosus Tissue and Cells of Different Degeneration Grades
- Ist Teil von
- International journal of molecular sciences, 2020-03, Vol.21 (6), p.2165
- Ort / Verlag
- Switzerland: MDPI AG
- Erscheinungsjahr
- 2020
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Intervertebral disc (IVD) herniation and degeneration is a major source of back pain. In order to regenerate a herniated and degenerated disc, closure of the anulus fibrosus (AF) is of crucial importance. For molecular characterization of AF, genome-wide Affymetrix HG-U133plus2.0 microarrays of native AF and cultured cells were investigated. To evaluate if cells derived from degenerated AF are able to initiate gene expression of a regenerative pattern of extracellular matrix (ECM) molecules, cultivated cells were stimulated with bone morphogenetic protein 2 (BMP2), transforming growth factor β1 (TGFβ1) or tumor necrosis factor-α (TNFα) for 24 h. Comparative microarray analysis of native AF tissues showed 788 genes with a significantly different gene expression with 213 genes more highly expressed in mild and 575 genes in severe degenerated AF tissue. Mild degenerated native AF tissues showed a higher gene expression of common cartilage ECM genes, whereas severe degenerated AF tissues expressed genes known from degenerative processes, including matrix metalloproteinases (MMP) and bone associated genes. During monolayer cultivation, only 164 differentially expressed genes were found. The cells dedifferentiated and altered their gene expression profile. RTD-PCR analyses of BMP2- and TGFβ1-stimulated cells from mild and severe degenerated AF tissue after 24 h showed an increased expression of cartilage associated genes. TNFα stimulation increased MMP1, 3, and 13 expression. Cells derived from mild and severe degenerated tissues could be stimulated to a comparable extent. These results give hope that regeneration of mildly but also strongly degenerated disc tissue is possible.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1422-0067, 1661-6596eISSN: 1422-0067DOI: 10.3390/ijms21062165Titel-ID: cdi_doaj_primary_oai_doaj_org_article_c5c4f526c62540ef9638a00525234503
- Format
- –
- Schlagworte
- Annulus Fibrosus - metabolism, Annulus Fibrosus - pathology, anulus fibrosus, Back pain, Bone matrix, Bone morphogenetic protein 2, Bone Morphogenetic Protein 2 - pharmacology, Cartilage, Cells, Cultured, Collagen, Cultivation, Degeneration, DNA microarrays, Extracellular matrix, Extracellular Matrix - genetics, Extracellular Matrix - metabolism, Gene expression, Gene Expression Profiling, Gene Expression Regulation - drug effects, Gene Expression Regulation - genetics, Genes, genome-wide microarray, Genomes, Growth factors, Humans, intervertebral disc, Intervertebral Disc - metabolism, Intervertebral Disc - pathology, Intervertebral Disc Degeneration - genetics, Intervertebral Disc Degeneration - metabolism, Intervertebral Disc Degeneration - pathology, Intervertebral Disc Displacement - genetics, Intervertebral Disc Displacement - metabolism, Intervertebral Disc Displacement - pathology, Intervertebral discs, Matrix metalloproteinase, Matrix Metalloproteinase 1 - genetics, Matrix Metalloproteinase 1 - metabolism, Matrix Metalloproteinase 13 - genetics, Matrix Metalloproteinase 13 - metabolism, Matrix Metalloproteinase 3 - genetics, Matrix Metalloproteinase 3 - metabolism, Matrix metalloproteinases, Oligonucleotide Array Sequence Analysis, Proteins, Regeneration, Regeneration - drug effects, Regeneration - genetics, Transforming Growth Factor beta1 - pharmacology, Tumor Necrosis Factor-alpha - pharmacology, Tumor necrosis factor-α