Details

Autor(en) / Beteiligte

• Anh, Nguyen To
• Nhu, Le Nguyen Truc
• Hong, Nguyen Thi Thu
• Phuc, Tran My
• Tam, Pham Thi Thanh
• Huong, Dang Thao
• Anh, Tran Tuan
• Deng, Xutao
• Nghia, Ho Dang Trung
• Nguyen, Tran Thua
• Van Hung, Nguyen
• Thuan, Nguyen Dac
• Phuong, Pham Thi Hong
• Chau, Nguyen Van Vinh
• Baker, Stephen
• Delwart, Eric
• Thwaites, Guy
• Van Tan, Le

Titel

Viral Metagenomic Analysis of Cerebrospinal Fluid from Patients with Acute Central Nervous System Infections of Unknown Origin, Vietnam

Ist Teil von

• Emerging infectious diseases, 2021-01, Vol.27 (1), p.205-213

Ort / Verlag

United States: U.S. National Center for Infectious Diseases

Erscheinungsjahr

2021

Quelle

MEDLINE

Beschreibungen/Notizen

• Central nervous system (CNS) infection is a serious neurologic condition, although the etiology remains unknown in >50% of patients. We used metagenomic next-generation sequencing to detect viruses in 204 cerebrospinal fluid (CSF) samples from patients with acute CNS infection who were enrolled from Vietnam hospitals during 2012-2016. We detected 8 viral species in 107/204 (52.4%) of CSF samples. After virus-specific PCR confirmation, the detection rate was lowered to 30/204 (14.7%). Enteroviruses were the most common viruses detected (n = 23), followed by hepatitis B virus (3), HIV (2), molluscum contagiosum virus (1), and gemycircularvirus (1). Analysis of enterovirus sequences revealed the predominance of echovirus 30 (9). Phylogenetically, the echovirus 30 strains belonged to genogroup V and VIIb. Our results expanded knowledge about the clinical burden of enterovirus in Vietnam and underscore the challenges of identifying a plausible viral pathogen in CSF of patients with CNS infections.