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Optimization of a Monobromobimane (MBB) Derivatization and RP-HPLC-FLD Detection Method for Sulfur Species Measurement in Human Serum after Sulfur Inhalation Treatment
Ist Teil von
Antioxidants, 2022-05, Vol.11 (5), p.939
Ort / Verlag
Switzerland: MDPI AG
Erscheinungsjahr
2022
Link zum Volltext
Quelle
Free E-Journal (出版社公開部分のみ)
Beschreibungen/Notizen
(1) Background: Hydrogen sulfide (H
S) is a widely recognized gasotransmitter, with key roles in physiological and pathological processes. The accurate quantification of H
S and reactive sulfur species (RSS) may hold important implications for the diagnosis and prognosis of diseases. However, H
S species quantification in biological matrices is still a challenge. Among the sulfide detection methods, monobromobimane (MBB) derivatization coupled with reversed phase high-performance liquid chromatography (RP-HPLC) is one of the most reported. However, it is characterized by a complex preparation and time-consuming process, which may alter the actual H
S level; moreover, a quantitative validation has still not been described. (2) Methods: We developed and validated an improved analytical protocol for the MBB RP-HPLC method. MBB concentration, temperature and sample handling were optimized, and the calibration method was validated using leave-one-out cross-validation and tested in a clinical setting. (3) Results: The method shows high sensitivity and allows the quantification of H
S species, with a limit of detection of 0.5 µM. Finally, it can be successfully applied in measurements of H
S levels in the serum of patients subjected to inhalation with vapors rich in H
S. (4) Conclusions: These data demonstrate that the proposed method is precise and reliable for measuring H
S species in biological matrices and can be used to provide key insights into the etiopathogenesis of several diseases and sulfur-based treatments.