Details

Autor(en) / Beteiligte

• Sharma, Nikhil
• Sen, Ganes C.

Titel

Intracranial delivery of antisense locked nucleic acids for silencing microRNAs in the mouse brain

Ist Teil von

• STAR protocols, 2022-12, Vol.3 (4), p.101836-101836, Article 101836

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2022

Quelle

MEDLINE

Beschreibungen/Notizen

• Antisense locked nucleic acid (LNA) technology has been widely used for silencing microRNAs with enhanced specificity and efficiency. In this protocol, we first describe the procedure for targeted intracranial delivery of LNAs to silence microRNAs specifically in the mouse brain. We then detail the steps to isolate RNA and protein from mouse brain, followed by using RT-PCR and Western blotting to confirm microRNA silencing. This noninvasive approach can only be applied to mouse brain to specifically target silencing of microRNAs. For complete details on the use and execution of this protocol, please refer to Sharma et al. (2021).1 [Display omitted] •Intracranial delivery of antisense LNAs•Noninvasive procedure to silence microRNAs specifically in mouse brain•Confirmation of silencing effect of LNAs on microRNAs Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Antisense locked nucleic acid (LNA) technology has been widely used for silencing microRNAs with enhanced specificity and efficiency. In this protocol, we first describe the procedure for targeted intracranial delivery of LNAs to silence microRNAs specifically in the mouse brain. We then detail the steps to isolate RNA and protein from mouse brain, followed by using RT-PCR and Western blotting to confirm microRNA silencing. This noninvasive approach can only be applied to mouse brain to specifically target silencing of microRNAs.