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International journal of molecular sciences, 2019-12, Vol.21 (1), p.145
2019
Details
- Autor(en) / Beteiligte
- Titel
- Gene Copy Number and Post-Transductional Mechanisms Regulate TRAP1 Expression in Human Colorectal Carcinomas
- Ist Teil von
- International journal of molecular sciences, 2019-12, Vol.21 (1), p.145
- Ort / Verlag
- Switzerland: MDPI AG
- Erscheinungsjahr
- 2019
- Link zum Volltext
- Quelle
- Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals
- Beschreibungen/Notizen
- Tumor Necrosis Factor Receptor-Associated Protein 1 (TRAP1) is a heat shock protein 90 (HSP90) molecular chaperone overexpressed in 60-70% human colorectal carcinomas (CRCs) and the co-upregulation of TRAP1 and associated 6-related proteins identifies metastatic CRCs with poor prognosis. Since the molecular mechanisms responsible for TRAP1 regulation are still unknown, the significance of gene copy number (CN) and the role of post-transductional protein modifications were addressed. gene aneuploidy accounted for 34.5% of cases in a cohort of 58 human CRCs and CN correlated with its mRNA and protein expression, suggesting that transcriptional mechanisms are responsible for TRAP1 upregulation. Furthermore, the analysis of the National Cancer Institute's Clinical Proteomic Tumor Analysis Consortium/The Cancer Genome Atlas (CPTAC/TCGA) CRC database showed that polysomy significantly correlates with lymph node involvement. However, a subgroup of tumors showed TRAP1 protein levels independent from its CN. Of note, a direct correlation was observed between TRAP1 protein levels and the expression of S-nitrosoglutathione reductase (GSNOR), a denitrosylase involved in the regulation of protein S-nitrosylation. Furthermore, CRC cell lines exposed to hypoxia or dichloroacetate treatment showed the downregulation of TRAP1 upon GSNOR silencing and this resulted in increased TRAP1 mono/polyubiquitination. These data suggest that transcriptional and post-transductional mechanisms account for TRAP1 expression in human CRCs and GSNOR protects TRAP1 from S-nitrosylation and consequent proteasome degradation mostly in conditions of stress.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1422-0067, 1661-6596eISSN: 1422-0067DOI: 10.3390/ijms21010145Titel-ID: cdi_doaj_primary_oai_doaj_org_article_c49bfe29f584480eb204861552a4b757
- Format
- –
- Schlagworte
- Adult, Aged, Aged, 80 and over, Aneuploidy, Biomarkers, Cancer, Cell cycle, Colorectal cancer, Colorectal carcinoma, Colorectal Neoplasms - genetics, Colorectal Neoplasms - metabolism, Consortia, Copy number, copy number variation, Correlation, Cyclin-dependent kinases, Dichloroacetic acid, Experiments, Female, Gene Dosage - genetics, Gene expression, Gene Expression Regulation, Neoplastic - genetics, Gene Expression Regulation, Neoplastic - physiology, Genomes, gsnor, Heat shock proteins, HSP90 Heat-Shock Proteins - genetics, HSP90 Heat-Shock Proteins - metabolism, Hsp90 protein, Humans, Hypotheses, Hypoxia, Kinases, Lymph nodes, Lymphatic system, Male, Medical prognosis, Metastases, Metastasis, Middle Aged, Molecular modelling, Proteasomes, Protein expression, Protein S, Proteins, Proteomics, Reductases, s-nitrosylation, Subgroups, Transcription, trap1, Tumors