Details

Autor(en) / Beteiligte

• Rossi, Átila Duque
• de Araújo, João Locke Ferreira
• de Almeida, Tailah Bernardo
• Ribeiro-Alves, Marcelo
• de Almeida Velozo, Camila
• Almeida, Jéssica Maciel de
• de Carvalho Leitão, Isabela
• Ferreira, Sâmila Natiane
• da Silva Oliveira, Jéssica
• Alves, Hugo José
• Scheid, Helena Toledo
• Faffe, Débora Souza
• Galliez, Rafael Mello
• de Ávila, Renata Eliane
• Resende, Gustavo Gomes
• Teixeira, Mauro Martins
• da Costa Ferreira Júnior, Orlando
• Castiñeiras, Terezinha Marta P P
• Souza, Renan Pedra
• Tanuri, Amilcar
• Aguiar, Renato Santana de
• Barroso, Shana Priscila Coutinho
• Cardoso, Cynthia Chester

Titel

Association between ACE2 and TMPRSS2 nasopharyngeal expression and COVID-19 respiratory distress

Ist Teil von

• Scientific reports, 2021-05, Vol.11 (1), p.9658-9658, Article 9658

Ort / Verlag

England: Nature Publishing Group

Erscheinungsjahr

2021

Link zum Volltext

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• ACE2 and TMPRSS2 are key players on SARS-CoV-2 entry into host cells. However, it is still unclear whether expression levels of these factors could reflect disease severity. Here, a case-control study was conducted with 213 SARS-CoV-2 positive individuals where cases were defined as COVID-19 patients with respiratory distress requiring oxygen support (N = 38) and controls were those with mild to moderate symptoms of the disease who did not need oxygen therapy along the entire clinical course (N = 175). ACE2 and TMPRSS2 mRNA levels were evaluated in nasopharyngeal swab samples by RT-qPCR and logistic regression analyzes were applied to estimate associations with respiratory outcomes. ACE2 and TMPRSS2 levels positively correlated with age, which was also strongly associated with respiratory distress. Increased nasopharyngeal ACE2 levels showed a protective effect against this outcome ( OR = 0.30; 95% CI 0.09-0.91), while TMPRSS2/ACE2 ratio was associated with risk ( OR = 4.28; 95% CI 1.36-13.48). On stepwise regression, TMPRSS2/ACE2 ratio outperformed ACE2 to model COVID-19 severity. When nasopharyngeal swabs were compared to bronchoalveolar lavages in an independent cohort of COVID-19 patients under mechanical ventilation, similar expression levels of these genes were observed. These data suggest nasopharyngeal TMPRSS2/ACE2 as a promising candidate for further prediction models on COVID-19.