Details
- Autor(en) / Beteiligte
- Titel
- In Vivo Targeting of Adult Neural Stem Cells in the Dentate Gyrus by a Split-Cre Approach
- Ist Teil von
- Stem cell reports, 2014-02, Vol.2 (2), p.153-162
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2014
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- We describe the labeling of adult neural stem cells (aNSCs) in the mouse and human dentate gyrus (DG) by the combinatorial expression of glial fibrillary acidic protein (GFAP) and Prominin1, as revealed by immunohistochemistry. Split-Cre-based genetic fate mapping of these double-positive cells in the adult murine DG reveals their NSC identity, as they are self-renewing and contribute to neurogenesis over several months. Their progeny reacts to stimuli such as voluntary exercise with increased neurogenesis. Prominin1+/GFAP+ cells also exist in the adult human DG, the only region in the human brain for which adult neurogenesis has been consistently reported. Our data, together with previous evidence of such double-positive NSCs in the developing murine brain and in neurogenic regions of vertebrates with widespread neurogenesis, suggest that Prominin1- and GFAP-expressing cells are NSCs in a wide range of species in development and adulthood. •Prominin1 is expressed in radial and nonradial NSCs in the adult hippocampus•Fate mapping reveals the long-term neurogenic lineage of Prominin1+/hGFAP+ NSCs•Prominin1 labels GFAP+ radial glia processes in the adult human hippocampus The combinatorial expression of GFAP and Prominin1 labels adult neural stem cells (NSCs) in the mouse dentate gyrus as revealed by immunohistochemistry and split-Cre-based fate mapping. Additionally, Prominin1+/GFAP+ cells exist in the adult human dentate gyrus. Such double-positive cells also represent NSCs in the embryonic forebrain and the adult subependymal zone, suggesting Prominin1+/GFAP+ expression as a general property of NSCs.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2213-6711eISSN: 2213-6711DOI: 10.1016/j.stemcr.2014.01.004Titel-ID: cdi_doaj_primary_oai_doaj_org_article_c3c6b02b5aa14cc78807513cb48ef011
- Format
- –
- Schlagworte
- AC133 Antigen, Adult Stem Cells - metabolism, Animals, Antigens, CD - genetics, Antigens, CD - metabolism, Dentate Gyrus - cytology, Dentate Gyrus - metabolism, Gene Expression, Gene Targeting, Genes, Reporter, Glial Fibrillary Acidic Protein - genetics, Glial Fibrillary Acidic Protein - metabolism, Glycoproteins - genetics, Glycoproteins - metabolism, Hippocampus - metabolism, Homologous Recombination, Humans, Mice, Neural Stem Cells - metabolism, Neuroglia - metabolism, Peptides - genetics, Peptides - metabolism, Protein Binding, Protein Transport