Details

Autor(en) / Beteiligte

• Mambuque, Edson
• Saavedra, Belén
• Molina-Moya, Barbara
• Nguenha, Dinis
• García-García, Esther
• Blanco, Silvia
• Gomes, Neide
• Ehrlich, Joanna
• Bulo, Helder
• Munguambe, Shilzia
• Chiconela, Helio
• Acacio, Sozinho
• Domínguez, José
• García-Basteiro, Alberto L

Titel

Evaluation of omnigene-sputum for preservation of sputum samples for diagnosis of 'Mycobacterium tuberculosis'

Ist Teil von

• Tropical medicine and infectious disease, 2023-07, Vol.8 (7), p.1-10

Ort / Verlag

Basel, Switzerland: MDPI

Erscheinungsjahr

2023

Link zum Volltext

Quelle

EZB Free E-Journals

Beschreibungen/Notizen

• In several low-income countries, the transport of sputa could take up to one week to reach the laboratories, resulting in increased contamination rates and a loss of growth. The aim of this study was to evaluate the effect of the OMNIgene-SPUTUM in preserving Mycobacterium tuberculosis on sputum samples simulating three hypothetical scenarios for conservation and/or decontamination: (1) sputum was mixed with OMN and conserved at room temperature for five days and then processed for culture (OMN); (2) sputum cultures followed the routine standing operating procedure at day 0 (STD); and (3) sputum samples were kept at room temperature for five days and mixed with the standard decontamination reagent (SDT5) and then processed for culture. The positivity rate based on smear microscopy was 36.4%, 29.1%, and 27.3% for STD, STD5, and OMN, respectively. The proportion of positive results by liquid culture (MGIT) was 39.1% (43/110) for STD, 26.4% (29/110) for STD5, and 20.0% for OMN (22/110). The overall concordance of liquid culture results was 51.8% (57/110): 37.3% (41/110) for negative results, 11.8% (13/110) for MTBC growth, and 2.7% (3/110) for contaminated results. The OMN arm showed better performance in solid culture than in liquid culture, with a notable reduction in contaminated results.