Details

Autor(en) / Beteiligte

• Li Mow Chee, Frederic
• Beernaert, Bruno
• Griffith, Billie G C
• Loftus, Alexander E P
• Kumar, Yatendra
• Wills, Jimi C
• Lee, Martin
• Valli, Jessica
• Wheeler, Ann P
• Armstrong, J Douglas
• Parsons, Maddy
• Leigh, Irene M
• Proby, Charlotte M
• von Kriegsheim, Alex
• Bickmore, Wendy A
• Frame, Margaret C
• Byron, Adam

Titel

Mena regulates nesprin-2 to control actin-nuclear lamina associations, trans-nuclear membrane signalling and gene expression

Ist Teil von

• Nature communications, 2023-03, Vol.14 (1), p.1602-1602, Article 1602

Ort / Verlag

England: Nature Publishing Group

Erscheinungsjahr

2023

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Interactions between cells and the extracellular matrix, mediated by integrin adhesion complexes, play key roles in fundamental cellular processes, including the sensing and transduction of mechanical cues. Here, we investigate systems-level changes in the integrin adhesome in patient-derived cutaneous squamous cell carcinoma cells and identify the actin regulatory protein Mena as a key node in the adhesion complex network. Mena is connected within a subnetwork of actin-binding proteins to the LINC complex component nesprin-2, with which it interacts and co-localises at the nuclear envelope. Moreover, Mena potentiates the interactions of nesprin-2 with the actin cytoskeleton and the nuclear lamina. CRISPR-mediated Mena depletion causes altered nuclear morphology, reduces tyrosine phosphorylation of the nuclear membrane protein emerin and downregulates expression of the immunomodulatory gene PTX3 via the recruitment of its enhancer to the nuclear periphery. We uncover an unexpected role for Mena at the nuclear membrane, where it controls nuclear architecture, chromatin repositioning and gene expression. Our findings identify an adhesion protein that regulates gene transcription via direct signalling across the nuclear envelope.