Nature communications, 2018-07, Vol.9 (1), p.2823-9, Article 2823
2018
Details
- Autor(en) / Beteiligte
- Titel
- Mapping the sensing spots of aerolysin for single oligonucleotides analysis
- Ist Teil von
- Nature communications, 2018-07, Vol.9 (1), p.2823-9, Article 2823
- Ort / Verlag
- England: Nature Publishing Group
- Erscheinungsjahr
- 2018
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Nanopore sensing is a powerful single-molecule method for DNA and protein sequencing. Recent studies have demonstrated that aerolysin exhibits a high sensitivity for single-molecule detection. However, the lack of the atomic resolution structure of aerolysin pore has hindered the understanding of its sensing capabilities. Herein, we integrate nanopore experimental results and molecular simulations based on a recent pore structural model to precisely map the sensing spots of this toxin for ssDNA translocation. Rationally probing ssDNA length and composition upon pore translocation provides new important insights for molecular determinants of the aerolysin nanopore. Computational and experimental results reveal two critical sensing spots (R220, K238) generating two constriction points along the pore lumen. Taking advantage of the sensing spots, all four nucleobases, cytosine methylation and oxidation of guanine can be clearly identified in a mixture sample. The results provide evidence for the potential of aerolysin as a nanosensor for DNA sequencing.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2041-1723eISSN: 2041-1723DOI: 10.1038/s41467-018-05108-5Titel-ID: cdi_doaj_primary_oai_doaj_org_article_c327e0aef98b4474928cf914bf232de3
- Format
- –
- Schlagworte
- Atomic structure, Bacterial Toxins - chemistry, Bases (nucleic acids), Binding Sites, Computer applications, Computer simulation, Cytosine, Deoxyribonucleic acid, Detection, DNA, DNA sequencing, DNA, Single-Stranded - chemistry, Experiments, Guanine, Lipid Bilayers - chemistry, Methylation, Molecular chains, Molecular Dynamics Simulation, Nanopores - ultrastructure, Nanotechnology - methods, Nucleic Acid Conformation, Oligonucleotides, Oligonucleotides - chemistry, Oxidation, Phosphatidylcholines - chemistry, Pore Forming Cytotoxic Proteins - chemistry, Porosity, Protein Binding, Protein Conformation, beta-Strand, Protein Interaction Domains and Motifs, Proteins, Sequence Analysis, DNA, Spots, Translocation