Details

Autor(en) / Beteiligte

• Tawfik, Rasha Gomaa
• Gawish, Mahmoud F
• Abotaleb, Mahmoud M
• Nada, Hassan S
• Morsy, Kareem
• Abumandour, Mohamed M A
• Torky, Helmy

Titel

Genetic Relationship between Salmonella Isolates Recovered from Calves and Broilers Chickens in Kafr El-Sheikh City Using ERIC PCR

Ist Teil von

• Animals (Basel), 2022-12, Vol.12 (23), p.3428

Ort / Verlag

Switzerland: MDPI AG

Erscheinungsjahr

2022

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• A prevalent bacterial intestinal infection with severe economic damage is salmonellosis. Our study was carried out to diagnose from chickens and calves, to determine its resistance to antimicrobials' phenotypic and genotypic characterization of integrons and β lactamase genes in the multidrug resistance of different serotypes, and to detect the genetic relationship between isolates collected from different origins using an ERIC PCR. In total, 200 samples from diseased chicken and diarrheic calves were obtained from 50 various farms from Kafr El-sheikh, Egypt. poultry isolates were characterized as (3/8), (3/8), and (2/8), but isolates from cattle were (1/2) and (1/2). When antibiotic susceptibility testing was completed on all of the isolates, it showed that there was multidrug resistance present (MDR). A PCR was applied for identifying the accompanying class 1 integrons and ESBLs from MDR isolates (two isolates of were divided as one from calf and one from poultry). Our results detected and class 1 , but were negative for , , and An ERIC PCR was conducted for understanding the clonal relation between various β-lactamase-producing MDR isolates. The same four previously mentioned isolates were also tested. The two isolates of isolated from poultry and calves had 100% similarity despite indicating that there were interactions between broilers and calves living on the same farm that caused infection from the same strains, while the other two isolates of showed only 33% serovarities.