Details

Autor(en) / Beteiligte

• Ma, Hongying
• Zhang, Han
• Deng, Jie
• Zhao, Hu
• Kong, Fei
• Jiang, Wei
• Zhang, Hongxing
• Dong, Xianggui
• Wang, Qijun

Titel

Detection the eDNA of Batrachuperus taibaiensis from the Zhouzhi Heihe River Using a Nested PCR Method and DNA Barcoding

Ist Teil von

• Animals (Basel), 2022-04, Vol.12 (9), p.1105

Ort / Verlag

Switzerland: MDPI AG

Erscheinungsjahr

2022

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• The Taibai stream salamander ( ) is a recently described species of the genus that occurs in the Zhouzhi Heihe River and is endangered in its native range. Here, we have established a method for water environmental DNA (eDNA) analysis of using a series of optimizations. We have designed a specific set of primers for the genus to amplify a 160 bp fragment of . The sequences were obtained from nested PCR on eDNA extracted from water samples, after which DNA barcoding was performed according to sequence analysis to determine the presence of the target species in the water. The method was validated using water from the Zhouzhi Heihe River with known populations and found that eDNA can move at least 150 m downstream from its point of origin. This study is the first to establish an optimal method for obtaining the eDNA of from water samples, which provides a theoretical basis for resource investigation and the protection of in future research. It is also an example of the eDNA extraction of other species that live in similar waters and are less genetically diverse between species.