Molecules (Basel, Switzerland), 2018-09, Vol.23 (9), p.2327
2018
Details
- Autor(en) / Beteiligte
- Titel
- Resveratrol Suppresses Matrix Metalloproteinase-2 Activation Induced by Lipopolysaccharide in Mouse Osteoblasts via Interactions with AMP-Activated Protein Kinase and Suppressor of Cytokine Signaling 1
- Ist Teil von
- Molecules (Basel, Switzerland), 2018-09, Vol.23 (9), p.2327
- Ort / Verlag
- Switzerland: MDPI AG
- Erscheinungsjahr
- 2018
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- ( ) lipopolysaccharide (LPS) is associated with the progression of bone resorption in periodontal and periapical diseases. Matrix metalloproteinase-2 (MMP-2) expression and activity are elevated in apical periodontitis and have been suggested to participate in bone resorption. Therefore, inhibiting MMP-2 activation may be considered a therapeutic strategy for treating apical periodontitis. Resveratrol is a natural non-flavonoid polyphenol that has been reported to have antioxidant, anti-cancer, and anti-inflammatory properties. However, the capacity of resveratrol to protect osteoblast cells from LPS insults and the mechanism of its inhibitory effects on MMP-2 activation is poorly understood. Here, we demonstrate that cell viability is unchanged when 10 mg L LPS is used, and MMP-2 expression is drastically induced by LPS in a concentration- and time-dependent manner. Twenty micromolar resveratrol did not reduce MC3T3-E1 cell viability. Resveratrol increased AMP-activated protein kinase (AMPK) phosphorylation, and Compound , a specific AMPK inhibitor, partially abolished the resveratrol-mediated phosphorylation of AMPK. In addition, AMPK inhibition blocked the effects of resveratrol on MMP-2 expression and activity in LPS-induced MC3T3-E1 cells. Treatment with resveratrol also induced suppressor of cytokine signaling 1 (SOCS1) expression in MC3T3-E1 cells. SOCS1 siRNA negated the inhibitory effects of resveratrol on LPS-induced MMP-2 production. Additionally, resveratrol-induced SOCS1 upregulation was reduced by treatment with compound . These results demonstrate that AMPK and SOCS1 activation are important signaling events during resveratrol-mediated inhibition of MMP-2 production in response to LPS in MC3T3-E1 cells, and there is crosstalk between AMPK and SOCS1 signaling.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1420-3049eISSN: 1420-3049DOI: 10.3390/molecules23092327Titel-ID: cdi_doaj_primary_oai_doaj_org_article_c04e5383db0a45a8b6974dc687d6884a
- Format
- –
- Schlagworte
- Activation, AMP-activated protein kinase, AMP-Activated Protein Kinases - genetics, AMP-Activated Protein Kinases - metabolism, Animals, apical periodontitis, Bacterial Proteins - adverse effects, Biomedical materials, Bone resorption, Cell death, cell signaling, Cell Survival - drug effects, Cell viability, Cytokines, Cytokines - metabolism, Dose-Response Relationship, Drug, Enzyme Activation - drug effects, Gelatinase A, Gelatinase B, Gene Expression Regulation - drug effects, Gum disease, Homeostasis, Inflammation, Interleukin 1, Kinases, lipopolysaccharide, Lipopolysaccharides, Lipopolysaccharides - adverse effects, Matrix metalloproteinase, Matrix Metalloproteinase 2 - genetics, Matrix Metalloproteinase 2 - metabolism, Matrix metalloproteinases, Metabolism, Metalloproteinase, Mice, Osteoblasts, Osteoblasts - cytology, Osteoblasts - drug effects, Osteoblasts - metabolism, Osteoclasts, Pain, Phosphorylation, Phosphorylation - drug effects, Porphyromonas endodontalis - metabolism, Protein expression, Proteins, Resveratrol, Resveratrol - pharmacology, Signal Transduction - drug effects, SOCS-1 protein, Suppressor of Cytokine Signaling 1 Protein - genetics, Suppressor of Cytokine Signaling 1 Protein - metabolism, Teeth, Time Factors, Toxicity