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Introgression of Black Rot Resistance from Brassica carinata to Cauliflower ( Brassica oleracea botrytis Group) through Embryo Rescue
Ist Teil von
Frontiers in plant science, 2017-07, Vol.8, p.1255-1255
Ort / Verlag
Switzerland: Frontiers Media S.A
Erscheinungsjahr
2017
Link zum Volltext
Quelle
EZB Electronic Journals Library
Beschreibungen/Notizen
Black rot caused by
pv.
(
) is a very important disease of cauliflower (
group) resulting into 10-50% yield losses every year. Since there is a dearth of availability of resistance to black rot disease in
(C genome), therefore exploration of A and B genomes was inevitable as they have been reported to be potential reservoirs of gene(s) for resistance to black rot. To utilize these sources, interspecific hybrid and backcross progeny (B
) were generated between cauliflower "Pusa Sharad" and Ethiopian mustard "NPC-9" employing
embryo rescue technique. Direct ovule culture method was better than siliqua culture under different temperature regime periods. Hybridity testing of F
inter-specific plants was carried out using co-dominant SSR marker and
B and C genome-specific (DB and DC) primers. Meiosis in the di-genomic (BCC) interspecific hybrid of
group (2
= 18, CC) ×
(2
= 4x = 34, BBCC) was higly disorganized and cytological analysis of pollen mother cells revealed chromosomes 2
= 26 at metaphase-I. Fertile giant pollen grain formation was observed frequently in interspecific F
hybrid and BC
plants. The F
inter-specific plants were found to be resistant to
race 1. Segregation distortion was observed in BC
generation for black rot resistance and different morphological traits. The At1g70610 marker analysis confirmed successful introgression of black rot resistance in interspecific BC
population. This effort will go a long way in pyramiding gene(s) for resistance against black rot in Cole crops, especially cauliflower and cabbage for developing durable resistance, thus minimize dependency on bactericides.