Details

Autor(en) / Beteiligte

• Jan, Taha A.
• Eltawil, Yasmin
• Ling, Angela H.
• Chen, Leon
• Ellwanger, Daniel C.
• Heller, Stefan
• Cheng, Alan G.

Titel

Spatiotemporal dynamics of inner ear sensory and non-sensory cells revealed by single-cell transcriptomics

Ist Teil von

• Cell reports (Cambridge), 2021-07, Vol.36 (2), p.109358-109358, Article 109358

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2021

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• The utricle is a vestibular sensory organ that requires mechanosensitive hair cells to detect linear acceleration. In neonatal mice, new hair cells are derived from non-sensory supporting cells, yet cell type diversity and mechanisms of cell addition remain poorly characterized. Here, we perform computational analyses on single-cell transcriptomes to categorize cell types and resolve 14 individual sensory and non-sensory subtypes. Along the periphery of the sensory epithelium, we uncover distinct groups of transitional epithelial cells, marked by Islr, Cnmd, and Enpep expression. By reconstructing de novo trajectories and gene dynamics, we show that as the utricle expands, Islr+ transitional epithelial cells exhibit a dynamic and proliferative phase to generate new supporting cells, followed by coordinated differentiation into hair cells. Taken together, our study reveals a sequential and coordinated process by which non-sensory epithelial cells contribute to growth of the postnatal mouse sensory epithelium. [Display omitted] •The neonatal mouse utricle contains five transcriptionally unique hair cell subtypes•Islr and Cnmd mark transitional epithelial cells•Transitional epithelial cells contribute to the expanding postnatal utricle•Computational trajectory analysis reveals gene dynamics of the expanding utricle The postnatal mouse utricle expands by more than 35% and doubles its number of hair cells during the first 8 days. Using single-cell transcriptomics, Jan et al. show that the surrounding transitional epithelial cells proliferate and contribute to the expansion of the sensory epithelium through a stepwise differentiation mechanism.