Nature communications, 2020-01, Vol.11 (1), p.495-495, Article 495
2020
Details
- Autor(en) / Beteiligte
- Titel
- A helitron-induced RabGDIα variant causes quantitative recessive resistance to maize rough dwarf disease
- Ist Teil von
- Nature communications, 2020-01, Vol.11 (1), p.495-495, Article 495
- Ort / Verlag
- England: Nature Publishing Group
- Erscheinungsjahr
- 2020
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Maize rough dwarf disease (MRDD), caused by various species of the genus Fijivirus, threatens maize production worldwide. We previously identified a quantitative locus qMrdd1 conferring recessive resistance to one causal species, rice black-streaked dwarf virus (RBSDV). Here, we show that Rab GDP dissociation inhibitor alpha (RabGDIα) is the host susceptibility factor for RBSDV. The viral P7-1 protein binds tightly to the exon-10 and C-terminal regions of RabGDIα to recruit it for viral infection. Insertion of a helitron transposon into RabGDIα intron 10 creates alternative splicing to replace the wild-type exon 10 with a helitron-derived exon 10. The resultant splicing variant RabGDIα-hel has difficulty being recruited by P7-1, thus leading to quantitative recessive resistance to MRDD. All naturally occurring resistance alleles may have arisen from a recent single helitron insertion event. These resistance alleles are valuable to improve maize resistance to MRDD and potentially to engineer RBSDV resistance in other crops.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2041-1723eISSN: 2041-1723DOI: 10.1038/s41467-020-14372-3Titel-ID: cdi_doaj_primary_oai_doaj_org_article_bd149929ce464d789b8d5396800dd989
- Format
- –
- Schlagworte
- Agricultural production, Alleles, Alternative splicing, Cereal crops, Cloning, Corn, Disease, Disease resistance, Disease Resistance - genetics, Domestication, Dwarf disease, Flowers & plants, Gene Expression Regulation, Plant, Genes, Genes, Plant, Genomes, Genomics, Guanine Nucleotide Dissociation Inhibitors - genetics, Guanine Nucleotide Dissociation Inhibitors - metabolism, Guanosine diphosphate, Infections, Inhibitors, Insertion, Laboratories, Models, Biological, Mutation, Physical Chromosome Mapping, Plant Diseases - genetics, Plant Diseases - virology, Plant Proteins - genetics, Plant Proteins - metabolism, Plant virus diseases, Plant Viruses - physiology, Plants, Genetically Modified, Protein Binding, Proteins, Quantitative Trait Loci - genetics, Reproducibility of Results, Rice, RNA, Messenger - genetics, RNA, Messenger - metabolism, Splicing, Transposons, Viral infections, Viral Proteins - metabolism, Viruses, Zea mays - genetics, Zea mays - ultrastructure, Zea mays - virology