Details

Autor(en) / Beteiligte

• Giotis, Efstathios S
• Laidlaw, Stephen M
• Bidgood, Susanna R
• Albrecht, David
• Burden, Jemima J
• Robey, Rebecca C
• Mercer, Jason
• Skinner, Michael A

Titel

Modulation of Early Host Innate Immune Response by an Avipox Vaccine Virus' Lateral Body Protein

Ist Teil von

• Biomedicines, 2020-12, Vol.8 (12), p.634

Ort / Verlag

Switzerland: MDPI

Erscheinungsjahr

2020

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• The avian pathogen fowlpox virus (FWPV) has been successfully used as a vaccine vector in poultry and humans, but relatively little is known about its ability to modulate host antiviral immune responses in these hosts, which are replication-permissive and nonpermissive, respectively. FWPV is highly resistant to avian type I interferon (IFN) and able to completely block the host IFN-response. Microarray screening of host IFN-regulated gene expression in cells infected with 59 different, nonessential FWPV gene knockout mutants revealed that FPV184 confers immunomodulatory capacity. We report that the -knockout virus (FWPVΔ184) induces the cellular IFN response as early as 2 h postinfection. The wild-type, uninduced phenotype can be rescued by transient expression of in FWPVΔ184-infected cells. Ectopic expression of inhibited polyI:C activation of the chicken IFN-β promoter and IFN-α activation of the chicken Mx1 promoter. Confocal and correlative super-resolution light and electron microscopy demonstrated that FPV184 has a functional nuclear localisation signal domain and is packaged in the lateral bodies of the virions. Taken together, these results provide a paradigm for a late poxvirus structural protein packaged in the lateral bodies, capable of suppressing IFN induction early during the next round of infection.