Details

Autor(en) / Beteiligte

• Cao, Kelei
• Liao, Xiang
• Lu, Jiahui
• Yao, Shu
• Wu, Fengjiao
• Zhu, Xingxing
• Shi, Dongyan
• Wen, Shuang
• Liu, Lixin
• Zhou, Hong

Titel

IL-33/ST2 plays a critical role in endothelial cell activation and microglia-mediated neuroinflammation modulation

Ist Teil von

• Journal of neuroinflammation, 2018-05, Vol.15 (1), p.136-136, Article 136

Ort / Verlag

England: BioMed Central Ltd

Erscheinungsjahr

2018

Quelle

MEDLINE

Beschreibungen/Notizen

• Interleukin-33 (IL-33) is increasingly being recognized as a key immunomodulatory cytokine in many neurological diseases. In the present study, wild-type (WT) and IL-33 mice received intracerebroventricular (i.c.v.) injection of lipopolysaccharide (LPS) to induce neuroinflammation. Intravital microscopy was employed to examine leukocyte-endothelial interactions in the brain vasculature. The degree of neutrophil infiltration was determined by myeloperoxidase (MPO) staining. Real-time PCR and western blotting were used to detect endothelial activation. Enzyme-linked immunosorbent assay and quantitative PCR were conducted to detect pro-inflammatory cytokine levels in the brain. In IL-33 mice, neutrophil infiltration in the brain cortex and leukocyte-endothelial cell interactions in the cerebral microvessels were significantly decreased as compared to WT mice after LPS injection. In addition, IL-33 mice showed reduced activation of microglia and cerebral endothelial cells. In vitro results indicated that IL-33 directly activated cerebral endothelial cells and promoted pro-inflammatory cytokine production in LPS-stimulated microglia. Our study indicated that IL-33/ST2 signaling plays an important role in the activation of microglia and cerebral endothelial cells and, therefore, is essential in leukocyte recruitment in brain inflammation. The role of IL-33/ST2 in LPS induced neuroinflammation.