International journal of molecular sciences, 2020-05, Vol.21 (10), p.3653
2020
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- Autor(en) / Beteiligte
- Titel
- Hydrostatic Pressure Regulates Oxidative Stress through microRNA in Human Osteoarthritic Chondrocytes
- Ist Teil von
- International journal of molecular sciences, 2020-05, Vol.21 (10), p.3653
- Ort / Verlag
- Switzerland: MDPI AG
- Erscheinungsjahr
- 2020
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Hydrostatic pressure (HP) modulates chondrocytes metabolism, however, its ability to regulate oxidative stress and microRNAs (miRNA) has not been clarified. The aim of this study was to investigate the role of miR-34a, miR-146a, and miR-181a as possible mediators of HP effects on oxidative stress in human osteoarthritis (OA) chondrocytes. Chondrocytes were exposed to cyclic low HP (1-5 MPa) and continuous static HP (10 MPa) for 3 hrs. Metalloproteinases (MMPs), disintegrin and metalloproteinase with thrombospondin motif (ADAMTS)-5, type II collagen (Col2a1), miR-34a, miR-146a, miR-181a, antioxidant enzymes, and B-cell lymphoma 2 (BCL2) were evaluated by quantitative real-time polymerase chain reaction qRT-PCR, apoptosis and reactive oxygen species ROS production by cytometry, and β-catenin by immunofluorescence. The relationship among HP, the studied miRNA, and oxidative stress was assessed by transfection with miRNA specific inhibitors. Low cyclical HP significantly reduced apoptosis, the gene expression of , , miRNA, the production of superoxide anion, and mRNA levels of antioxidant enzymes. Conversely, an increased and genes was observed. β-catenin protein expression was reduced in cells exposed to HP 1-5 MPa. Opposite results were obtained following continuous static HP application. Finally, miRNA silencing enhanced low HP and suppressed continuous HP-induced effects. Our data suggest miRNA as one of the mechanisms by which HP regulates chondrocyte metabolism and oxidative stress, via Wnt/β-catenin pathway.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1422-0067, 1661-6596eISSN: 1422-0067DOI: 10.3390/ijms21103653Titel-ID: cdi_doaj_primary_oai_doaj_org_article_bafa030e82684755b2d67eb8cc972b15
- Format
- –
- Schlagworte
- ADAMTS5 Protein - genetics, ADAMTS5 Protein - metabolism, Aged, Antioxidants, Apoptosis, B-cell lymphoma, beta Catenin - genetics, beta Catenin - metabolism, Biomedical materials, Cartilage, Cell adhesion & migration, Cells, Cultured, Chondrocytes, Chondrocytes - metabolism, Collagen (type II), Collagen Type II - genetics, Collagen Type II - metabolism, Collagenase 3, Cytometry, Enzymes, Female, Gene expression, Humans, Hydrostatic Pressure, Immunofluorescence, Lymphocytes B, Male, Matrix Metalloproteinase 13 - genetics, Matrix Metalloproteinase 13 - metabolism, Matrix metalloproteinases, Metabolism, microRNA, MicroRNAs, MicroRNAs - genetics, MicroRNAs - metabolism, miRNA, Osteoarthritis, Osteoarthritis - genetics, Osteoarthritis - metabolism, Oxidative metabolism, Oxidative Stress, Physiology, Polymerase chain reaction, Pressure, Proto-Oncogene Proteins c-bcl-2 - genetics, Proto-Oncogene Proteins c-bcl-2 - metabolism, Superoxide, Thrombospondin, Transfection, Tumor necrosis factor-TNF, Wnt protein, Wnt Signaling Pathway, Wnt/β-catenin, β-Catenin