Details

Autor(en) / Beteiligte

• Kanber, Deniz
• Woestefeld, Julia
• Döpper, Hannah
• Bozet, Morgane
• Brenzel, Alexandra
• Altmüller, Janine
• Kilpert, Fabian
• Lohmann, Dietmar
• Pommerenke, Claudia
• Steenpass, Laura

Titel

RB1 -Negative Retinal Organoids Display Proliferation of Cone Photoreceptors and Loss of Retinal Differentiation

Ist Teil von

• Cancers, 2022-04, Vol.14 (9), p.2166

Ort / Verlag

Switzerland: MDPI AG

Erscheinungsjahr

2022

Link zum Volltext

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Retinoblastoma is a tumor of the eye in children under the age of five caused by biallelic inactivation of the tumor suppressor gene in maturing retinal cells. Cancer models are essential for understanding tumor development and in preclinical research. Because of the complex organization of the human retina, such models were challenging to develop for retinoblastoma. Here, we present an organoid model based on differentiation of human embryonic stem cells into neural retina after inactivation of by CRISPR/Cas9 mutagenesis. Wildtype and heterozygous mutant retinal organoids were indistinguishable with respect to morphology, temporal development of retinal cell types and global mRNA expression. However, loss of pRB resulted in spatially disorganized organoids and aberrant differentiation, indicated by disintegration of organoids beyond day 130 of differentiation and depletion of most retinal cell types. Only cone photoreceptors were abundant and continued to proliferate, supporting these as candidate cells-of-origin for retinoblastoma. Transcriptome analysis of knockout organoids and primary retinoblastoma revealed gain of a retinoblastoma expression signature in the organoids, characterized by upregulation of (p107), , , and . In addition, genes related to immune response and extracellular matrix were specifically upregulated in -negative organoids. In vitro retinal organoids therefore display some features associated with retinoblastoma and, so far, represent the only valid human cancer model for the development of this disease.