Details
- Autor(en) / Beteiligte
- Titel
- Evolutionary routes to biochemical innovation revealed by integrative analysis of a plant-defense related specialized metabolic pathway
- Ist Teil von
- eLife, 2017-08, Vol.6
- Ort / Verlag
- England: eLife Science Publications, Ltd
- Erscheinungsjahr
- 2017
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The diversity of life on Earth is a result of continual innovations in molecular networks influencing morphology and physiology. Plant specialized metabolism produces hundreds of thousands of compounds, offering striking examples of these innovations. To understand how this novelty is generated, we investigated the evolution of the Solanaceae family-specific, trichome-localized acylsugar biosynthetic pathway using a combination of mass spectrometry, RNA-seq, enzyme assays, RNAi and phylogenomics in different non-model species. Our results reveal hundreds of acylsugars produced across the Solanaceae family and even within a single plant, built on simple sugar cores. The relatively short biosynthetic pathway experienced repeated cycles of innovation over the last 100 million years that include gene duplication and divergence, gene loss, evolution of substrate preference and promiscuity. This study provides mechanistic insights into the emergence of plant chemical novelty, and offers a template for investigating the ~300,000 non-model plant species that remain underexplored.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2050-084XeISSN: 2050-084XDOI: 10.7554/elife.28468Titel-ID: cdi_doaj_primary_oai_doaj_org_article_ba8f3f3385ed448fbae50c3353c66a7d
- Format
- –
- Schlagworte
- Acylation, Acyltransferases - genetics, Acyltransferases - metabolism, Analysis, Base Sequence, Biological Evolution, Biosynthesis, Capsicum, Carbohydrate Metabolism - genetics, Carbohydrate Metabolism - physiology, Convolvulaceae, Defense industry, Diseases, Divergence, enzyme evolution, Enzymes, Evolution, Evolution, Molecular, Gene Amplification, Gene duplication, Gene Knockdown Techniques, Gene Silencing, Genes, Genetic research, Genomics and Evolutionary Biology, Innovations, Instrument industry (Equipment), Mass Spectrometry, Mass spectroscopy, Metabolic Networks and Pathways, Metabolism, Metabolites, Novelty, Petunia, phylogenetic reconstruction, Phylogenetics, Phylogeny, Physiological aspects, Plant Biology, plant defense, Plant metabolites, Plant Proteins - genetics, Plant Proteins - metabolism, Promiscuity, Proteins, Ribonucleic acid, RNA, RNA interference, RNA, Plant, RNA-mediated interference, Solanaceae, Solanaceae - classification, Solanaceae - enzymology, Solanaceae - genetics, Solanaceae - metabolism, Solanum, Solanum lycopersicum, specialized metabolism, Spectroscopy, Substrate Specificity, Sucrose, Sucrose - metabolism, Sugar, Sugars - chemistry, Sugars - metabolism, Time, Tobacco (Plant), Transcriptome, trichome, Trichomes - enzymology, Trichomes - genetics, Trichomes - metabolism