Scientific reports, 2021-09, Vol.11 (1), p.19400-16, Article 19400
2021
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- Autor(en) / Beteiligte
- Titel
- Adipose expression of CREB3L3 modulates body weight during obesity
- Ist Teil von
- Scientific reports, 2021-09, Vol.11 (1), p.19400-16, Article 19400
- Ort / Verlag
- England: Nature Publishing Group
- Erscheinungsjahr
- 2021
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- We found the hepatic transcription factor Cyclic-AMP Responsive Element Binding Protein 3-like-3 (CREB3L3) to be expressed in adipose tissue, and selectively downregulated in the more metabolically protective subcutaneous adipose tissue in obese mice and humans. We sought to elucidate the specific role of this factor in adipose biology. CREB3L3 fat-specific knockout mice were fed a high-fat diet to induce obesity and metabolic dysfunction. Additionally, we injected a flip-excision adeno-associated virus directly into the subcutaneous inguinal adipose tissue of Adiponectin-Cre mice to create a depot-specific overexpression model for further assessment. Fat-specific ablation of CREB3L3 enhanced weight gain and insulin resistance following high-fat feeding, as fat-specific knockout mice expended less energy and possessed more inflammatory adipose tissue. Conversely, inguinal fat CREB3L3 overexpression deterred diet-induced obesity and ameliorated metabolic dysfunction. Together, this study highlights the relevance of CREB3L3 in obese adipose tissue and demonstrates its role as a powerful body weight modulator.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2045-2322eISSN: 2045-2322DOI: 10.1038/s41598-021-98627-zTitel-ID: cdi_doaj_primary_oai_doaj_org_article_ba282b53acb742e59a203873b579f767
- Format
- –
- Schlagworte
- Adiponectin, Adipose tissue, Adolescent, Adult, Aged, Aged, 80 and over, Animals, Body weight, Body weight gain, Cyclic AMP Response Element-Binding Protein - metabolism, Cyclic AMP Response Element-Binding Protein - pharmacology, Female, High fat diet, Humans, Inflammation, Insulin, Insulin resistance, Male, Metabolism, Mice, Mice, Inbred C57BL, Mice, Knockout, Mice, Obese, Middle Aged, Obesity, Obesity - drug therapy, Obesity - metabolism, Subcutaneous Fat - drug effects, Subcutaneous Fat - metabolism, Young Adult