Details

Autor(en) / Beteiligte

• Sohrab, Sayed Sartaj
• Madani, Tariq Ahmed
• Kafrawy, Sherif Ali El
• Al-Bar, Hussein Mohamed
• elzein, Altayeb Mohamed Abo
• Farraj, Suha Abdulaal Ali
• Uthman, Norah Abdulhamed
• Hassan, Ahmed Mohamed Sharif
• Bajrai, Leena Hussein
• Azhar, Esam Ibraheem

Titel

Full-genome sequencing and analysis of DENV-3 serotype isolated from Yemen

Ist Teil von

• Journal of infection and public health, 2021-06, Vol.14 (6), p.803-810

Ort / Verlag

Elsevier Ltd

Erscheinungsjahr

2021

Quelle

EZB-FREE-00999 freely available EZB journals

Beschreibungen/Notizen

• Dengue virus causes the dengue fever as well as hemorrhagic fever in tropical and sub-tropical countries. It is now endemic in most parts of the South East Asia. Full-genome information of dengue virus 3 is not available from Yemen. In this study, the dengue virus 3 was detected by diagnostic tools like serology and RT-PCR in the samples isolated from a patient in Yemen. The full-genome was sequenced, and the identity, phylogenetic relationship and recombination analysis was performed by using BioEdit, MEGA X and RDP4 softwares. The full-genome of the Yemen isolate was found to be 10,643 nt long with 3390 amino acids. The Yemen dengue virus 3 isolate showed the sequence similarity (98.5–92.4%) with dengue virus 3 isolates from China, Pakistan, India and Bangladesh respectively. The significant non-synonymous substitutions of amino acid in Yemen isolate were observed with selected isolates. The phylogenetic tree of Yemen isolate formed a unique clade within genotype III and sub-clade into lineage III. The Dengue virus isolate from Jeddah formed separated cluster with lineage IV. This reveals the unique genetic variability among DENV-3 serotypes from Jeddah and earlier reported isolates from other regions.