Details

Autor(en) / Beteiligte

• Jiménez, Isabel
• Tazón-Vega, Bárbara
• Abrisqueta, Pau
• Nieto, Juan C
• Bobillo, Sabela
• Palacio-García, Carles
• Carabia, Júlia
• Valdés-Mas, Rafael
• Munuera, Magdalena
• Puigdefàbregas, Lluís
• Parra, Genís
• Esteve-Codina, Anna
• Franco-Jarava, Clara
• Iacoboni, Gloria
• Terol, María José
• García-Marco, José Antonio
• Crespo, Marta
• Bosch, Francesc

Titel

Immunological and genetic kinetics from diagnosis to clinical progression in chronic lymphocytic leukemia

Ist Teil von

• Biomarker research, 2021-05, Vol.9 (1), p.37-37, Article 37

Ort / Verlag

England: BioMed Central Ltd

Erscheinungsjahr

2021

Link zum Volltext

Quelle

Springer Nature - Complete Springer Journals

Beschreibungen/Notizen

• Mechanisms driving the progression of chronic lymphocytic leukemia (CLL) from its early stages are not fully understood. The acquisition of molecular changes at the time of progression has been observed in a small fraction of patients, suggesting that CLL progression is not mainly driven by dynamic clonal evolution. In order to shed light on mechanisms that lead to CLL progression, we investigated longitudinal changes in both the genetic and immunological scenarios. We performed genetic and immunological longitudinal analysis using paired primary samples from untreated CLL patients that underwent clinical progression (sampling at diagnosis and progression) and from patients with stable disease (sampling at diagnosis and at long-term asymptomatic follow-up). Molecular analysis showed limited and non-recurrent molecular changes at progression, indicating that clonal evolution is not the main driver of clinical progression. Our analysis of the immune kinetics found an increasingly dysfunctional CD8 T cell compartment in progressing patients that was not observed in those patients that remained asymptomatic. Specifically, terminally exhausted effector CD8 T cells (T-bet Eomes PD1 ) accumulated, while the the co-expression of inhibitory receptors (PD1, CD244 and CD160) increased, along with an altered gene expression profile in T cells only in those patients that progressed. In addition, malignant cells from patients at clinical progression showed enhanced capacity to induce exhaustion-related markers in CD8 T cells ex vivo mainly through a mechanism dependent on soluble factors including IL-10. Altogether, we demonstrate that the interaction with the immune microenvironment plays a key role in clinical progression in CLL, thereby providing a rationale for the use of early immunotherapeutic intervention.