Nature communications, 2020-08, Vol.11 (1), p.4269-4269, Article 4269
2020
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- Autor(en) / Beteiligte
- Titel
- Complete chemical structures of human mitochondrial tRNAs
- Ist Teil von
- Nature communications, 2020-08, Vol.11 (1), p.4269-4269, Article 4269
- Ort / Verlag
- England: Nature Publishing Group
- Erscheinungsjahr
- 2020
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Mitochondria generate most cellular energy via oxidative phosphorylation. Twenty-two species of mitochondrial (mt-)tRNAs encoded in mtDNA translate essential subunits of the respiratory chain complexes. mt-tRNAs contain post-transcriptional modifications introduced by nuclear-encoded tRNA-modifying enzymes. They are required for deciphering genetic code accurately, as well as stabilizing tRNA. Loss of tRNA modifications frequently results in severe pathological consequences. Here, we perform a comprehensive analysis of post-transcriptional modifications of all human mt-tRNAs, including 14 previously-uncharacterized species. In total, we find 18 kinds of RNA modifications at 137 positions (8.7% in 1575 nucleobases) in 22 species of human mt-tRNAs. An up-to-date list of 34 genes responsible for mt-tRNA modifications are provided. We identify two genes required for queuosine (Q) formation in mt-tRNAs. Our results provide insight into the molecular mechanisms underlying the decoding system and could help to elucidate the molecular pathogenesis of human mitochondrial diseases caused by aberrant tRNA modifications.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2041-1723eISSN: 2041-1723DOI: 10.1038/s41467-020-18068-6Titel-ID: cdi_doaj_primary_oai_doaj_org_article_b76731e827284bb38227d56d00e3395f
- Format
- –
- Schlagworte
- Acidosis, Amino acids, Bases (nucleic acids), Cardiomyopathy, Cellular structure, Decoding, Electron transport, Enzymes, Female, Gene sequencing, Genes, Genetic Code, Genomes, HEK293 Cells, HeLa Cells, Humans, Laboratories, Mass Spectrometry, Mass spectroscopy, Mitochondria, Mitochondria - metabolism, Mitochondria - pathology, Mitochondrial Diseases - genetics, Mitochondrial Diseases - pathology, Mitochondrial DNA, Molecular modelling, Molecular Structure, Mutation, Nucleoside Q - biosynthesis, Nucleoside Q - chemistry, Oxidative Phosphorylation, Pathogenesis, Phosphorylation, Placenta, Post-transcription, Pregnancy, Protein synthesis, Proteins, Queues, Queuosine, Ribonucleic acid, RNA, RNA Processing, Post-Transcriptional, RNA, Mitochondrial - chemistry, RNA, Mitochondrial - isolation & purification, RNA, Mitochondrial - metabolism, RNA, Transfer - chemistry, RNA, Transfer - isolation & purification, RNA, Transfer - metabolism, RNA-Seq, Species, tRNA